National Institutes of Health, AR53579, Ostéogenèse: Potentialisation avec peptides Tropic os National Institutes of Health, AR056422, effet anabolisant de peptides ostéogénique National Institutes of Health, T32 AR050960, réparation musculo-squelettiques et la régénération DOD, PC051219 nucléoline: Un médiateur Roman de cancer de la prostate et la moelle osseuse d&#39;adhésion cellulaire endothéliale Département de la Défense, PC061508, cancer de la prostate Métastases osseuses Cell-médiateurs adhérence moelle

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La production de cette vidéo a été parrainé par New England Biolabs, Inc, qui produit certains des réactifs utilisés dans cet article.

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peptides from phage display library modulate gene expression in mesenchymal cells and potentiate osteogenesis in unicortical bone defects

Deux nouveaux peptides synthétiques d&#39;accélérer la formation osseuse et peut être livré en utilisant une matrice de collagène. L&#39;objectif de cette étude était d&#39;étudier les effets sur la réparation osseuse dans un modèle de défauts unicortical. Le traitement des cellules mésenchymateuses produit une augmentation de l&#39;activité phosphatase alcaline, a montré la formation de nodules par les cellules, et augmenté l&#39;expression de gènes codant pour RUNX2, osterix, sialoprotéine osseuse et l&#39;ostéocalcine. Une éponge de collagène imbibée de réparation promu peptide de défauts osseux, tandis que le contrôle a été moins efficace. Les résultats de cette étude ont démontré que les cellules mésenchymateuses traités avec le peptide in vitro de différenciation vers l&#39;ostéogenèse, et que les peptides livré in vivo en utilisant une éponge de collagène favoriser la réparation des défauts unicortical.

Watch this Scientific Journal Video about Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects at JoVE.com

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Une bibliothèque d&#39;affichage de phages a été utilisé pour identifier les séquences peptidiques qui os cible. L&#39;objectif était d&#39;étudier l&#39;effet de ces peptides sur la différenciation des cellules mésenchymateuses et de déterminer leur effet sur la régénération osseuse.

Les peptides de la bibliothèque de Phage Display modulent l&#39;expression génétique dans les cellules mésenchymateuses et potentialiser l&#39;ostéogenèse dans des défauts osseux Unicortical

Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects ...

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17.9K vues.  University of Virginia. Une bibliothèque d'affichage de phages a été utilisé pour identifier les séquences peptidiques qui os cible. L'objectif était d'étudier l'effet de ces peptides sur la différenciation des cellules mésenchymateuses et de déterminer leur effet sur la régénération osseuse.