Ce travail a été soutenu par une subvention de l&#39;Institut national de l&#39;arthrite et les maladies cutanées musculo-squelettiques, le NIH RO1AR054458.

1. Étiquetage des hMSCs avec Endorem <ol><li> Les cellules sont cultivées à 80% de confluence d&#39;au moins 18 heures avant l&#39;étiquetage. </li><li> Pendant ce temps, les médias étiquetage est préparé en ajoutant à l&#39;échantillon Endorem à une dose de 100 ug Fe / ml du milieu sans sérum. </li><li> Après les cellules sont cultivées jusqu&#39;à confluence, la culture médiatique est aspirée et les cellules ne sont lavées avec du PBS 1x ou un milieu sans sérum. </li><li> Ensuite, la solution de rinçage est aspiré et les médias d&#39;étiquetage préalablement préparé est ajouté. Les cellules sont ensuite incubées à 37 ° C, 5% de CO 2 pendant 4 heures. </li><li> Après 4 heures d&#39;incubation, l&#39;étiquetage des médias est aspiré et les cellules sont rincées avec du PBS et la trypsine, comme d&#39;habitude. </li><li> Une fois trypsinées, les cellules sont lavées 3x avec PBS et centrifugés à 400rcf pendant 5 minutes. </li><li> Après cette étape de lavage, les cellules sont remises en suspension, compté, évalué pour la viabilité, et utilisés au besoin pour l&#39;expérimentation. </li></ol> 2. Préparation d&#39;agarose <ol><li> Après que les cellules ont été marqués, il est temps pour préparer la solution d&#39;agarose. </li><li> Sur une échelle plus fine, de 80 mg d&#39;agarose en poudre sont pesés et ajoutés à 2 ml de PBS. </li><li> Cette solution est ensuite agitée doucement et autoclavés. </li><li> Après environ 40 min, la solution fluide agarose peuvent être retirés de l&#39;autoclave. Le volume devrait être mesurée et ajustée en conséquence avec préchauffé PBS. </li><li> Puis la solution est refroidie à 42 ° C </li></ol> 3. Créer MASI <ol><li> Lorsque la solution d&#39;agarose a atteint la température désirée, les cellules sont comptées et centrifugé pour la dernière fois dans un tube Eppendorf de 1.5 ml. </li><li> Après centrifugation, le surnageant est éliminé et les cellules sont mélangées avec le DMEM pour atteindre une concentration de 30 x 10 6 cellules / ml. </li><li> Maintenant, les cellules sont mélangées avec de l&#39;agarose 42 ° C au chaud. </li><li> Une pointe de la pipette froid peut entraîner agarose à solidifier l&#39;intérieur de la pointe, il est donc conseillé de préchauffer le bout par pipetage chaude PBS stérile haut et en bas </li><li> Maintenant, le même volume d&#39;agarose comme support est repris et soigneusement mélangé avec la cellule-media-suspension ainsi que d&#39;une suspension homogène est créé. </li><li> Garder le tube Eppendorf dans un bain d&#39;eau préchauffée, tout en mélangeant afin que l&#39;agarose ne refroidit pas assez pour se solidifier. </li><li> Lorsque la suspension est homogène, les cellules sont implantées dans le vice. </li></ol><img src="/files/ftp_upload/1793/1793fig1.jpg" alt="Figure 1" /> Figure 1. Coronale pondérée en T2 images SE (TR 4000 ms / TE 18,27 ms) d&#39;un spécimen de la rotule avec hMSCs implanté dans les lésions du cartilage.

<ol>
	<li>Mauck, R. L., Yuan, X., Tuan, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chondrogenic+differentiation+and+functional+maturation+of+bovine+mesenchymal+stem+cells+in+long-term+agarose+culture.">Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture.</a> Osteoarthritis Cartilage. 14, 179-189 (2006).</li><li>Pittenger, M. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multilineage+potential+of+adult+human+mesenchymal+stem+cells.">Multilineage potential of adult human mesenchymal stem cells.</a> Science. 284, 143-147 (1999).</li><li>Rahfoth, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantation+of+allograft+chondrocytes+embedded+in+agarose+gel+into+cartilage+defects+of+rabbits.">Transplantation of allograft chondrocytes embedded in agarose gel into cartilage defects of rabbits.</a> Osteoarthritis Cartilage. 6, 50-65 (1998).</li><li>Henning, T. D., Boddington, S., Daldrup-Link, H. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Labeling+hESCs+and+hMSCs+with+iron+oxide+nanoparticles+for+non-invasive+in+vivo+tracking+with+MR+imaging.">Labeling hESCs and hMSCs with iron oxide nanoparticles for non-invasive in vivo tracking with MR imaging.</a> J Vis Exp. , (2008).</li></ol>

Le protocole décrit fournit une manière reproductible à l&#39;étiquette MSC avec des nanoparticules d&#39;oxyde de fer et implanter ces étiquetés MSC dans des défauts du cartilage. Cette technique permet non invasive représentation de greffes de cellules souches à des défauts du cartilage en IRM, ce qui permet une détection précoce de l&#39;échec MASI. Une luxation ou d&#39;efflux des cellules marquées peuvent être diagnostiqués à partir d&#39;une disparition de l&#39;étiquette à partir du site de tra...

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<td>D-MEM High Glucose</td>
<td>Reagent</td>
<td>Sigma</td>
<td>D5648</td>
<td>&nbsp;</td>
<tr>
<td>PBS (Ca++, Mg++ free)</td>
<td>&nbsp;</td>
<td>GIBCO</td>
<td>14190-144</td>
<td>&nbsp;</td>
<tr>
<td>Trypsin-EDTA 0.05%</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>25399-120</td>
<td>&nbsp;</td>
<tr>
<td>FBS</td>
<td>&nbsp;</td>
<td>Hyclone</td>
<td>SH30071.03</td>
<td>&nbsp;</td>
<tr>
<td>Penicillin/Streptomycin</td>
<td>&nbsp;</td>
<td>GIBCO</td>
<td>15140-122</td>
<td>&nbsp;</td>
<tr>
<td>Ferumoxides (Endorem)</td>
<td>
</td>
<td>Guerbet</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
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<td>Agarose Typ VII</td>
<td>&nbsp;</td>
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<td>A9045</td>
<td>Dilute Agarose in PBS to final concentration of 4%</td>
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implantation of ferumoxides labeled human mesenchymal stem cells in cartilage defects

Le domaine du génie tissulaire intègre les principes de l&#39;ingénierie, la biologie cellulaire et en médecine vers la régénération des cellules spécifiques et des tissus fonctionnels. Matrice des implants de cellules souches associées (MASI) visent à régénérer les défauts du cartilage due à l&#39;arthrite ou de lésions traumatiques commune. Adulte cellules souches mésenchymateuses (CSM) ont la capacité de se différencier en cellules de la lignée chondrocytaire et ont montré des résultats prometteurs pour les technologies basées sur les cellules articulaires réparation du cartilage. Autologue MSC peut être isolé d&#39;une variété de tissus, peut être étendu dans les cultures cellulaires sans perdre leur potentiel de différenciation, et ont démontré la différenciation chondrocytaire in vitro et in vivo 1, 2. Afin d&#39;assurer la rétention locale et la viabilité des cellules souches mésenchymateuses transplantées dans les lésions du cartilage, un échafaudage est nécessaire, qui prend également en charge de différenciation et la prolifération subséquente. L&#39;architecture de la formation des guides et des tissus échafaudage permet la matrice extracellulaire, produite par les cellules souches, à se développer. Des recherches antérieures ont montré que 2% d&#39;agarose échafaudage peut soutenir le développement du cartilage hyalin stable et ne pas induire des réponses immunitaires 3. Conservation à long terme des cellules souches transplantées dans le MASI est essentiel pour la régénération du cartilage. Étiquetage des CSM avec des nanoparticules d&#39;oxyde de fer permet à long terme dans le suivi in vivo avec des techniques non invasives d&#39;imagerie IRM 4. Cette présentation fera la démonstration des techniques de MSC étiquetage avec des nanoparticules d&#39;oxyde de fer, la génération de cellules-agarose des constructions et l&#39;implantation de ces constructions dans des défauts du cartilage. Les constructions étiquetées peuvent être suivis de façon non invasive avec MR-imagerie.

Watch this Scientific Journal Video about Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects at JoVE.com

Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Objectif de la présentation est de montrer une méthode hautement reproductible pour générer la matrice associée implants de cellules souches dans les lésions du cartilage, qui peuvent être visualisées avec l&#39;IRM. Les cellules souches sont étiquetés avec approuvée par la FDA Ferumoxides, mélangés avec l&#39;agarose, implantés dans les lésions du cartilage et imagé avec un scanner IRM 7T.

Implantation de Ferumoxides Étiqueté cellules souches mésenchymateuses humaines dans les lésions du cartilage

The field of tissue engineering integrates the principles of engineering, cell biology and medicine towards the regeneration of specific cells and ...

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10.5K vues.  Medical Center, University of California San Francisco. Objectif de la présentation est de montrer une méthode hautement reproductible pour générer la matrice associée implants de cellules souches dans les lésions du cartilage, qui peuvent être visualisées avec l'IRM. Les cellules souches sont étiquetés avec approuvée par la FDA Ferumoxides, mélangés avec l'agarose, implantés dans les lésions du cartilage et imagé avec un scanner IRM 7T.

Vidéo: Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

The umbilical cord is a rich source for progenitor cells with high proliferative potential including mesenchymal stromal cells (also termed mesenchymal stem cells, MSCs) and endothelial colony forming progenitor cells (ECFCs). Both cell types are key players in maintaining the integrity of tissue and are probably also involved in regenerative processes and tumor formation.
To study their biology and function in a comparative manner it is important to have both cells types available from the same donor. It may also be beneficial for regenerative purposes to derive MSCs and ECFCs from the same tissue.
Because cellular therapeutics should eventually find their way from bench to bedside we established a new method to isolate and further expand progenitor cells without the use of animal protein. Pooled human platelet lysate (pHPL) replaced fetal bovine serum in all steps of our protocol to completely avoid contact of the cells to xenogeneic proteins.
This video demonstrates a methodology for the isolation and expansion of progenitor cells from one umbilical cord.
All materials and procedures will be described.

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells MSCs and Endothelial Colony Forming Progenitor Cells ECFCs

This protocol describes the isolation and subsequent expansion of mesenchymal stromal cells and endothelial colony forming cells without the use of animal serum to generate autologous pairs for experimental transplantation purposes.

Isolement et animale expansion sans sérum du cordon ombilical humain dérivé cellules stromales mésenchymateuses (CSM) et des cellules endothéliales progénitrices Colony Forming (CPEF)

The umbilical cord is a rich source for progenitor cells with high proliferative potential including mesenchymal stromal cells (also termed mesenchymal ...

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Biologie

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects on bone repair in a unicortical defect model. Treatment of mesenchymal cells produced an increase in alkaline phosphatase activity, showed nodule formation by the cells, and increased the expression of genes for runx2, osterix, bone sialoprotein, and osteocalcin. A collagen sponge soaked with peptide promoted repair of bone defects, whereas the control was less effective. The results from this study demonstrated that mesenchymal cells treated with peptide in vitro differentiate towards osteogenesis, and, that peptides delivered in vivo using a collagen sponge promote the repair of unicortical defects.

A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of these peptides on mesenchymal cell differentiation and to determine their effect on bone regeneration.

Les peptides de la bibliothèque de Phage Display modulent l&#39;expression génétique dans les cellules mésenchymateuses et potentialiser l&#39;ostéogenèse dans des défauts osseux Unicortical

Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects ...

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Human embryonic stem cells (hESCs) are pluripotent and an invaluable cellular sources for in vitro disease modeling and regenerative medicine1. It has been previously shown that human somatic cells can be reprogrammed to pluripotency by ectopic expression of four transcription factors (Oct4, Sox2, Klf4 and Myc) and become induced pluripotent stem cells (iPSCs)2-4 . Like hESCs, human iPSCs are pluripotent and a potential source for autologous cells. Here we describe the protocol to reprogram human fibroblast cells with the four reprogramming factors cloned into GFP-containing retroviral backbone4. Using the following protocol, we generate human iPSCs in 3-4 weeks under human ESC culture condition. Human iPSC colonies closely resemble hESCs in morphology and display the loss of GFP fluorescence as a result of retroviral transgene silencing. iPSC colonies isolated mechanically under a fluorescence microscope behave in a similar fashion as hESCs. In these cells, we detect the expression of multiple pluripotency genes and surface markers.

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP

A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.

Reprogrammation des cellules somatiques humaines dans cellules souches pluripotentes induites (CISP) Utilisation vecteur rétroviral avec la GFP

Human embryonic stem cells (hESCs) are pluripotent and an invaluable cellular sources for in vitro disease modeling and regenerative medicine1. It has ...

Induction and Analysis of Epithelial to Mesenchymal Transition

Epithelial to mesenchymal transition (EMT) is essential for proper morphogenesis during development. Misregulation of this process has been implicated as a key event in fibrosis and the progression of carcinomas to a metastatic state. Understanding the processes that underlie EMT is imperative for the early diagnosis and clinical control of these disease states. Reliable induction of EMT in vitro is a useful tool for drug discovery as well as to identify common gene expression signatures for diagnostic purposes. Here we demonstrate a straightforward method for the induction of EMT in a variety of cell types. Methods for the analysis of cells pre- and post-EMT induction by immunocytochemistry are also included. Additionally, we demonstrate the effectiveness of this method through antibody-based array analysis and migration/invasion assays.

A straightforward method is described for the induction of epithelial to mesenchymal transition (EMT) in a variety of cell types. Methods for the analysis of cells in EMT states by immunocytochemistry are included.

Induction et analyse des épithéliale à mésenchymateuses transition

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

Understanding the cellular and molecular mechanisms that underlie tooth regeneration and renewal has become a topic of great interest1-4, and the mouse incisor provides a model for these processes. This remarkable organ grows continuously throughout the animal&#39;s life and generates all the necessary cell types from active pools of adult stem cells housed in the labial (toward the lip) and lingual (toward the tongue) cervical loop (CL) regions. Only the dental stem cells from the labial CL give rise to ameloblasts that generate enamel, the outer covering of teeth, on the labial surface. This asymmetric enamel formation allows abrasion at the incisor tip, and progenitors and stem cells in the proximal incisor ensure that the dental tissues are constantly replenished. The ability to isolate and grow these progenitor or stem cells in vitro allows their expansion and opens doors to numerous experiments not achievable in vivo, such as high throughput testing of potential stem cell regulatory factors. Here, we describe and demonstrate a reliable and consistent method to culture cells from the labial CL of the mouse incisor.

The continuously growing mouse incisor provides a model for studying renewal of dental tissues from dental epithelial stem cells (DESCs). A robust system for consistently and reliably obtaining these cells from the incisor and expanding them in vitro is reported here.

Isolement et culture des cellules souches épithéliales dentaire de l&#39;adulte de souris Incisive

Understanding the cellular and molecular mechanisms that underlie tooth regeneration and renewal has become a topic of great interest1-4, and the mouse ...

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

A few years ago, the establishment of human induced pluripotent stem cells (iPSCs) ushered in a new era in biomedicine. Potential uses of human iPSCs include modeling pathogenesis of human genetic diseases, autologous cell therapy after gene correction, and personalized drug screening by providing a source of patient-specific and symptom relevant cells. However, there are several hurdles to overcome, such as eliminating the remaining reprogramming factor transgene expression after human iPSCs production. More importantly, residual transgene expression in undifferentiated human iPSCs could hamper proper differentiations and misguide the interpretation of disease-relevant in vitro phenotypes. With this reason, integration-free and/or transgene-free human iPSCs have been developed using several methods, such as adenovirus, the piggyBac system, minicircle vector, episomal vectors, direct protein delivery and synthesized mRNA. However, efficiency of reprogramming using integration-free methods is quite low in most cases.
Here, we present a method to isolate human iPSCs by using Sendai-virus (RNA virus) based reprogramming system. This reprogramming method shows consistent results and high efficiency in cost-effective manner.

Here, we present our established method to reprogram human somatic cells into transgene-free human iPSCs with Sendai virus, which shows consistent outcome and enhanced efficiency.

Génération efficace d&#39;origine humaine souches pluripotentes à partir de cellules somatiques humaines avec Sendai-virus

A few years ago, the establishment of human induced pluripotent stem cells (iPSCs) ushered in a new era in biomedicine. Potential uses of human iPSCs ...

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Heterogeneity of stem cell population hampers detailed understanding of stem cell biology, such as their differentiation propensity toward different lineages. A single cell transcriptome assay can be a new approach for dissecting individual variation. We have developed the single cell qRT-PCR method, and confirmed that this method works well in several gene expression profiles. In single cell level, each human embryonic stem cell, sorted by OCT4::EGFP positive cells, has high expression in OCT4, but a different level of NANOG expression. Our single cell gene expression assay should be useful to interrogate population heterogeneities.

Single cell gene expression assay is needed for understanding stem cell heterogeneities.

Profilage individuel embryonnaires humaines Cellules souches par RT-PCR quantitative

Heterogeneity of stem cell population hampers detailed understanding of stem cell biology, such as their differentiation propensity toward different ...

A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells

The ultracentrifugation-based process is considered the common method for small extracellular vesicles (sEVs) isolation. However, the yield from this isolation method is relatively lower, and these methods are inefficient in separating sEV subtypes. This study demonstrates a simple benchtop filtration method to isolate human umbilical cord-derived MSC small extracellular vesicles (hUC-MSC-sEVs), successfully separated by ultrafiltration from the conditioned medium of hUC-MSCs. The size distribution, protein concentration, exosomal markers (CD9, CD81, TSG101), and morphology of the isolated hUC-MSC-sEVs were characterized with nanoparticle tracking analysis, BCA protein assay, western blot, and transmission electron microscope, respectively. The isolated hUC-MSC-sEVs&#39; size was 30-200 nm, with a particle concentration of 7.75 &#215; 1010 particles/mL and a protein concentration of 80 &#181;g/mL. Positive bands for exosomal markers CD9, CD81, and TSG101 were observed. This study showed that hUC-MSC-sEVs were successfully isolated from hUC-MSCs conditioned medium, and characterization showed that the isolated product fulfilled the criteria mentioned by Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV 2018).

This protocol demonstrates how to isolate human umbilical cord-derived mesenchymal stem cells&#39; small extracellular vesicles (hUC-MSC-sEVs) with a simple lab-scale benchtop setting. The size distribution, protein concentration, sEVs markers, and morphology of isolated hUC-MSC-sEVs are characterized by nanoparticle tracking analysis, BCA protein assay, western blot, and transmission electron microscope, respectively.

Une méthode de filtration de paillasse simple pour isoler de petites vésicules extracellulaires à partir de cellules souches mésenchymateuses humaines

The ultracentrifugation-based process is considered the common method for small extracellular vesicles (sEVs) isolation. However, the yield from this ...

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Extracellular vesicles (EVs) are heterogeneous membrane nanoparticles released by most cell types, and they are increasingly recognized as physiological regulators of organismal homeostasis and important indicators of pathologies; in the meantime, their immense potential to establish accessible and controllable disease therapeutics is emerging. Mesenchymal stem cells (MSCs) can release large amounts of EVs in culture, which have shown promise to jumpstart effective tissue regeneration and facilitate extensive therapeutic applications with good scalability and reproducibility. There is a growing demand for simple and effective protocols for collecting and applying MSC-EVs. Here, a detailed protocol is provided based on differential centrifugation to isolate and characterize representative EVs from cultured human MSCs, exosomes, and microvesicles for further applications. The adaptability of this method is shown for a series of downstream approaches, such as labeling, local transplantation, and systemic injection. The implementation of this procedure will address the need for simple and reliable MSC-EVs collection and application in translational research.

The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.

Isolement, caractérisation et application thérapeutique de vésicules extracellulaires à partir de cellules souches mésenchymateuses humaines en culture

Extracellular vesicles (EVs) are heterogeneous membrane nanoparticles released by most cell types, and they are increasingly recognized as physiological ...

Isolement et caractérisation de cellules souches dérivées de tissu adipeux

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Adult mesenchymal cells have revolutionized molecular and cell biology in recent decades. They can differentiate into different specialized cell types, in addition to their great capacity for self-renewal, migration, and proliferation. Adipose tissue is one of the least invasive and most accessible sources of mesenchymal cells. It has also been reported to have higher yields compared to other sources, as well as superior immunomodulatory properties. Recently, different procedures for obtaining adult mesenchymal cells from different tissue sources and animal species have been published. After evaluating the criteria of some authors, we standardized a methodology that is applicable to different purposes and easily reproducible. A pool of stromal vascular fraction (SVF) from perirenal and epididymal adipose tissue allowed us to develop primary cultures with optimal morphology and functionality. The cells were observed adhered to the plastic surface for 24 h, and exhibited a fibroblast-like morphology, with prolongations and a tendency to form colonies. Flow cytometry (FC) and immunofluorescence (IF) techniques were used to assess the expression of the membrane markers CD105, CD9, CD63, CD31, and CD34. The ability of adipose-derived stem cells (ASCs) to differentiate into the adipogenic lineage was also assessed using a cocktail of factors (4 &#181;M insulin, 0.5 mM 3-methyl-iso-butyl-xanthine, and 1 &#181;M dexamethasone). After 48 h, a gradual loss of fibroblastoid morphology was observed, and at 12 days, the presence of lipid droplets positive to oil red staining was confirmed. In summary, a procedure is proposed to obtain optimal and functional ASC cultures for application in regenerative medicine.

Pleins feux sur l’auteur : Isolement et identification de cellules souches mésenchymateuses dérivées du tissu adipeux de rats Sprague Dawley  

This protocol describes a methodology for isolating and identifying adipose tissue-derived mesenchymal stem cells (MSCs) from Sprague Dawley rats.

Isolement et identification de cellules souches mésenchymateuses dérivées du tissu adipeux de rats Sprague Dawley

Adult mesenchymal cells have revolutionized molecular and cell biology in recent decades. They can differentiate into different specialized cell types, in ...