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Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

DOI :

10.3791/2690-v

April 29th, 2011

April 29th, 2011

15,879 Views

1Department of Biomedical Engineering, University of Wisconsin – Madison

The following protocol outlines the process of isolating, ventilating and instrumenting mouse lungs to measure steady or pulsatile pulmonary vascular pressure-flow relationships in order to quantify the effects of blood flow, airflow, airway changes and vascular changes on right ventricular afterload.

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