S'identifier

JoVE Journal

Medicine

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Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

12.2K Views

10:50 min

June 29th, 2013

June 29th, 2013

12,212 Views

0:05

Title

2:47

Priming the Osmotic Pump

3:58

Creating Unilateral Hindlimb Ischemia in the Mouse

6:30

Imaging Specimen Preparation

9:40

Results: Representative Perfusion of the Vasculature in the Murine Hindlimb Ischemia Model

10:27

Conclusion

We show here the in vivo insertion of an osmotic pump for constant local drug delivery and the creation of hindlimb ischemia in a mouse model. Moreover, the hindlimb vasculature is perfused with Microfil, a silicone radiopaque agent, to prepare for micro-computed tomography (micro-CT) imaging.

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