March 11th, 2014
•The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.
Vidéos Connexes
Fertilization of Xenopus oocytes using the Host Transfer Method (Video) | JoVE
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation (Video) | JoVE
Mouse Oocyte Microinjection, Maturation and Ploidy Assessment (Video) | JoVE
Single Oocyte Bisulfite Mutagenesis (Video) | JoVE
Study of the DNA Damage Checkpoint using Xenopus Egg Extracts (Video) | JoVE
Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging (Video) | JoVE
Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4 (Video) | JoVE
Xenopus laevis as a Model to Identify Translation Impairment (Video) | JoVE
Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products (Video) | JoVE
Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy (Video) | JoVE