March 13th, 2014
•The described comparative, quantitative proteomic approach aims at obtaining insights into the composition of multiprotein complexes under different conditions and is demonstrated by comparing genetically different strains. For quantitative analysis equal volumes of different fractions from a sucrose density gradient are mixed and analyzed by mass spectrometry.
Vidéos Connexes
MALDI Sample Preparation: the Ultra Thin Layer Method Video (Video) | JoVE
A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry Video (Video) | JoVE
Analyzing Large Protein Complexes by Structural Mass Spectrometry Video (Video) | JoVE
BN-PAGE: Blue Native-Polyacrylamide Gel Electrophoresis BN-PAGE: Blue Native-Polyacrylamide Gel Electrophoresis | Protocol (Video) | JoVE
Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation Video (Video) | JoVE
Protein Interactions: Metabolic Labeling,Co-Immunoprecipitation, Quantitative Mass Spec and Affinity Modulation | Protocol (Video) | JoVE
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures Video (Video) | JoVE
A New Technique for Quantitative Analysis of Hair Loss in Mice Using Grayscale Analysis Video (Video) | JoVE
Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry Video (Video) | JoVE
Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels Video (Video) | JoVE