March 9th, 2015
•Described here is a rapid and effective procedure for functional reconstitution of purified wild-type and mutant CFTR protein that preserves activity for this chloride channel, which is defective in Cystic Fibrosis. Iodide efflux from reconstituted proteoliposomes mediated by CFTR allows studies of channel activity and the effects of small molecules.
Vidéos Connexes
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid Video (Video) | JoVE
Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding Video (Video) | JoVE
Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity Video (Video) | JoVE
Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein GST-RhoAG17A from Epithelial Cell Lysates Video (Video) | JoVE
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease Video (Video) | JoVE
Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies Video (Video) | JoVE
Demonstration of Proteolytic Activation of the Epithelial Sodium Channel ENaC by Combining Current Measurements with Detection of Cleavage Fragments Video (Video) | JoVE
One-channel Cell-attached Patch-clamp Recording Video (Video) | JoVE
In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae Video (Video) | JoVE
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions Video (Video) | JoVE