December 12th, 2015
•The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.
Vidéos Connexes
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures
Visualizing Stromule Frequency with Fluorescence Microscopy
Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel
Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy