Preparation of Gel, Infrared Fluorescent Dye-labeled Probes and Unlabeled Probes
4:49
Binding Reaction and Electrophoresis
6:37
Imaging
7:35
Results: Electrophoretic Mobility Shift Assays Using Purified Proteins and Dye-labeled DNA Probes
8:57
Conclusion
Transcription
The overall goal of this assay is to detect protein-DNA interactions using non-radioactive probes. This method can help answer key questions in the molecular biology and biochemistry fields, such as determining DNA target sequence of proteins. The
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
We describe here an optimized protocol of fluorescent Electrophoretic Mobility Shift Assays (fEMSA) using purified SOX-2 proteins together with infrared fluorescent dye-labeled DNA probes as a case study to tackle an important biological question.