Preparative HPLC Purification of the Aβ40 or Aβ42 Peptide
2:17
Purification of Aβ Peptide
3:26
Analytical HPLC Analysis of the Purified Aβ Protein
4:42
Results: HPLC Measurements of Purified Aβ42 Peptide
5:53
Conclusion
Transcription
The overall goal of this procedure is to use HPLC purification to produce high purity amyloid beta 42, and amyloid beta 40 peptides that are capable of forming oligomers.The biochemical properties of the amyloid beta peptide can often make its pur
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Herein we report a tailored HPLC purification protocol that yields high-purity amyloid beta 42 (Aβ42) and amyloid beta 40 (Aβ40) peptides, capable of oligomer formation. Amyloid beta is a highly aggregation prone, hydrophobic peptide implicated in Alzheimer's disease. The amyloidogenic nature of the peptide makes its purification a challenge.