The overall goal of this experiment is to stage pupal periods and measure wing pigmentation of Drosophila guttifera. This method can help answer key questions in the evolutionary developmental biology field, such as how pigmentation patterns are d
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Protocols for staging pupal periods and measurement of wing pigmentation of Drosophila guttifera are described. Staging and quantification of pigmentation provide a solid basis for studying developmental mechanisms of adult traits and enable interspecific comparison of trait development.