Construction of Mesh Sample Baskets and 24-well Basket Holder
3:37
In Situ Hybridization
6:25
Results: Tips for Success
8:05
Conclusion
Transcription
This method can help to answer key questions about tick entomology, such as assessing the presence of viable bacteria in the tick spatially and temporally. The main advantages of this technique are that it does not require sectioning of the ticks
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Here, we present a protocol to spatially and temporally assess the presence of viable microbiota in tick guts using a modified whole-mount in situ hybridization approach.