exfoliation-analysis-large-area-air-sensitive-two-dimensional

Exfoliation and Analysis of Large-area, Air-Sensitive Two-Dimensional Materials

We describe methods for producing and analyzing large, thin flakes of air-sensitive two-dimensional materials. Thin flakes of layered or van der Waals ...

the-automated-crystallography-pipelines-at-embl-htx-facility

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

EMBL Grenoble operates the High Throughput Crystallization Laboratory (HTX Lab), a large-scale user facility offering high throughput crystallography ...

University of Arkansas

SUNY Geneseo

European Molecular Biology Laboratory

Currently, macromolecular crystallography projects often require the use of highly automated facilities for crystallization and X-ray data collection. However, crystal harvesting and processing largely depend on manual operations. Here, a series of new methods are presented based on the use of a low X-ray-background film as a crystallization support and a photoablation laser that enable the automation of major operations required for the preparation of crystals for X-ray diffraction experiments. In this approach, the controlled removal of the mother liquor before crystal mounting simplifies the cryocooling process, in many cases eliminating the use of cryoprotectant agents, while crystal-soaking experiments are performed through diffusion, precluding the need for repeated sample-recovery and transfer operations. Moreover, the high-precision laser enables new mounting strategies that are not accessible through other methods. This approach bridges an important gap in automation and can contribute to expanding the capabilities of modern macromolecular crystallography facilities. 

Peter Murphy

Department of Physics

University of Arkansas

Exfoliation and Analysis of Large-area, Air-Sensitive Two-Dimensional Materials

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

Peter Murphy

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Physics

University of Arkansas

Exfoliation and Analysis of Large-area, Air-Sensitive Two-Dimensional Materials

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

Department of Physics