Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Murine Ommaya Assembly and Implantation

1. Preparation of station
   1. Disinfect the station surface. Place a blue covering over the surface and keep ready all sterilized tools and supplies indicated in Figure 1.
2. Presurgical procedure
   1. Apply analgesia (Bup-SR) and maintain sterile surgical environment.
3. Surgical implantation of Murine Ommaya
   1. Assemble the Murine Ommaya inje....

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תוצאות

Figure 1: An example of a workstation setup for performing the Murine Ommaya implantation in mice. (1) Gas anesthesia machine/vaporizer. (2) Sterile blue paper drape covering a stereotaxic stand. (3) Stereotaxic device (stand/stage, ear bars, nose cone). (4) Microdrill. (5) Magnifying glass with light. (6) Sterile cotton-tipped applicator sticks with sterile saline rinse container. (7) Hydrogen peroxide. (8) Bead sterilizer.

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Materials

Name	Company	Catalog Number	Comments
1 mm spacer disc	Alzet, Durect Corporation	#0008670	Spacer disc only
4-0 ethilon nylon suture	Any vendor
Automatic syringe pumps	Harvard Syringe Pumps (or any vendor)	#70-4505	Pump 11 Elite
Bead sterilizer	Braintree Scientific Inc. (or any vendor)	#GER 5287-120V	Germinator 500
Buprenorphine Sustained-Release (Bup-SR)	Zoopharm		DEA controlled
Cyanoacrylate sterile adhesive	Any vendor
Gas inhalation anestehsia system	VeteEquip	#901812	COMPAC5
Hamilton microliter syringes	Hamilton		10, 25, 50, and 100 μL; 30G for cisterna magna injection
Hydrogen peroxide	Any vendor
IVIS 200 imaging system	Caliper Life Sciences
Magnifying glass with light	Any vendor
Microdrill	Stoelting (or any vendor)	#51555M
MRI imaging	Bruker	BioSpec series	Optional
Murine Ommaya (MRI-compatible) prototype	Instech Laboratories, Inc.	#VAB620-25MRI-3.3
PinPort injector	Instech Laboratories, Inc.	#PNP3M-50
PinPort	Instech Laboratories, Inc.	#1-PNP3F28-50
Rodent Surgical Instruments (Scissors, Forceps)	Roboz Surgical Instrument (or any vendor)
Stereotaxic device	Stoelting (or any vendor)	#51730M
Sterile blue paper/ drape covering	Any vendor
Sterile cotton sticks	Any vendor