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Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain
Overview
This video demonstrates ommaya mediated intracerebroventricular injection in a mouse model. The method enables localized delivery of potential drug candidates and evades the blood-brain barrier.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Murine Ommaya Assembly and Implantation
- Preparation of station
- Disinfect the station surface. Place a blue covering over the surface and keep ready all sterilized tools and supplies indicated in Figure 1.
- Presurgical procedure
- Apply analgesia (Bup-SR).......
Representative Results
Figure 1: An example of a workstation setup for performing the Murine Ommaya implantation in mice. (1) Gas anesthesia machine/vaporizer. (2) Sterile blue paper drape covering a stereotaxic stand. (3) Stereotaxic device (stand/stage, ear bars, nose cone). (4) Microdrill. (5) Magnifying glass with light. (6) Sterile cotton-tipped applicator sticks with sterile saline rinse container. (7) Hydrogen peroxide. (8) Bead sterilizer.
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Source: Law, V. et al. A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease. J. Vis. Exp. (2021)
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