מחקר זה מומן על ידי &quot;הקרן המדעית לרפואה של סרן של העיר וינה &#39;ו Biosurgery Ethicon, Inc ניו ג&#39;רזי, ארה&quot;ב. המחברים מביעים את תודתם פרופ Heber Ferraz-Leite, מנהל &quot;סדנת האירופי microsurgery ו מוחין revascularization &#39;באוניברסיטה הרפואית של וינה, אוסטריה, להוראה microsurgical יקר שלו.

1. מיקום והכנת החיה הניסויים אושרו על ידי הוועדה האחראית האתי המקומי ביצע על פי הנחיות Felasa. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> התחל הרדמה כללית של הנקבה ניו זילנד ארנב לבן (2.5-3.5 ק&quot;ג) עם זריקה תוך שרירית של Ketamin (30mg/kg) ו Xylazine (6mg/kg) ולהמשיך לאחר אינטובציה מכני (קוטר צינור 4 מ&quot;מ, אורך 18 מ&quot;מ) עם גז הרדמה ( ) Isoflurane תחת ניטור אק&quot;ג. ביצוע ניטור הכאב באמצעות צביטה הבוהן כל 15 דקות ולהתאים הרדמה במקרה של תשובה חיובית. </li><li style=";text-align:right;direction:rtl"> תקן ארנב במצב פרקדן על צלחת bodywarm. </li><li style=";text-align:right;direction:rtl"> הכינו את אזור הניתוח על ידי הסרת שיער לכת מזווית הלסת עד אמצע החזה. ביצוע כל ההליכים בתנאים סטריליים. </li></ol> 2. הכנת עורק הצוואר חיצוני <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> ביצוע חתך קו האמצע מזווית הלסת עד sterni manubrium. </li><li style=";text-align:right;direction:rtl"> באמצעות מיקרוסקופ הניתוח, בחר קטע 1 ס&quot;מ של וריד הצוואר השמאלי חיצוני ללא סניפים ורידי ולבודד אותו microsurgically (איור 1 א). במהלך המבצע כולו לעתים קרובות להחיל 4% papaverin פתרון HCl פתרון אנטיביוטי (5mg/ml neomycin סולפט) מקומי על כלי שיט anastomoses כדי למנוע זיהומים vasospasm ומקומיים. </li><li style=";text-align:right;direction:rtl"> ולקשור את הווריד proximally ו distally עם התפרים polyfilament 4-0 (Vicryl, Ethicon Inc, ניו ג&#39;רזי, ארה&quot;ב), ואז להסירו ולשמור אותו מלוחים heparinized (1000 הפרין IU ב 20 מ&quot;ל תמיסת מלח 0.9% ו - 1 מ&quot;ל 4% papaverin HCl ). </li></ol> 3. הכנה של שני עורקי הראש הנפוצים (CCAs) <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> הכן קטע של המרכז לאמנות עכשווית שמאל להגיע מ הסתעפות הראש למטה קשת אבי העורקים. לשמר ככל האפשר סניפים עורקי רץ מדיאלית ואספקת מבנים בגרון ואת קנה הנשימה, כמו גם כל המבנים העצביים. </li><li style=";text-align:right;direction:rtl"> הכינו את המרכז לאמנות עכשווית הנכון. גיוס זה עד הסתעפות הצוואר ומטה כדי המטען bracchiocephalic. </li><li style=";text-align:right;direction:rtl"> ניהול הפרין 1000 IU לווריד. </li><li style=";text-align:right;direction:rtl"> קליפ המרכז לאמנות עכשווית זכות זמנית, ממש מתחת הסתעפות הצוואר. </li><li style=";text-align:right;direction:rtl"> ולקשור CCA ימין proximally ישירות מעל תא המטען brachiocephalic. חותכים את המרכז לאמנות עכשווית ממש מעל המיתר ולהשקות את הגדם עם מלוחים heparinized. </li><li style=";text-align:right;direction:rtl"> גזור לכרות בזהירות את adventitia בסוף חינם של המרכז לאמנות עכשווית הנכון. חינם בקפידה קטע של המרכז לאמנות עכשווית שמאל מתוכננת השקה של adventitia קליפ זה distally ו proximally. </li></ol> 4. השקה של שני CCAs <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> בצע arteriotomy אליפטי של המרכז לאמנות עכשווית שמאל בין קליפים לפי גודל של השקה המתוכנן עם המרכז לאמנות עכשווית ימין כיס ורידי. להשקות את המרכז לאמנות עכשווית שמאל עם מלוחים heparinized. (איור 1b) </li><li style=";text-align:right;direction:rtl"> תפר היקף האחורי של זכות המרכז לאמנות עכשווית, הגדם לתוך arteriotomy של המרכז לאמנות עכשווית שמאל, באמצעות 4-5 ללא תפרים monofilament resorbable 10-0 (10-0 Ethilon, Ethicon Inc, ניו ג&#39;רזי, ארה&quot;ב). </li><li style=";text-align:right;direction:rtl"> ביצוע חתך אורכי של גדם של המרכז לאמנות עכשווית הזכות להתאים את היקף חצי כיס ורידי. Anastomose את הצד האחורי של קיר הנרתיק ורידי הראשון עם arteriotomy של המרכז לאמנות עכשווית שמאל, שוב באמצעות 4-5 10-0 התפרים. ואז anastomose את הצד האחורי של כיס ורידי עם הישבן של המרכז לאמנות עכשווית ימין עם 3-4 תפרים. (איור 1 ג&#39;) </li><li style=";text-align:right;direction:rtl"> ביצוע השקה באותו סדר בצד הקדמי. (איור 1d) </li><li style=";text-align:right;direction:rtl"> הסר את הקליפ דיסטלי על המרכז לאמנות עכשווית הנכון. מפרצת היא בדרך כלל לא אטום לחלוטין, ולפיכך לשטוף אוויר לכוד החוצה פסולת. </li><li style=";text-align:right;direction:rtl"> חותם את קווי תפר סביב השקה לבין צוואר מפרצת עם בלוק שומן דבק הפיברין (Evicel, Ethicon Biosurgery בע&quot;מ, ניו ג&#39;רזי, ארה&quot;ב). הסרת קליפים הנותרים. </li><li style=";text-align:right;direction:rtl"> השתמש התפרים resorbable 4-0 (Monocryl, Ethicon Inc, ניו ג&#39;רזי, ארה&quot;ב) עבור קטע תפרים תת עורית ואחר כך תפר cutaneus פועל. </li></ol> 5. לאחר הניתוח וניהול <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> ניהול 10 מ&quot;ג / ק&quot;ג חומצה אצטילסליצילית לתוך הווריד, 60 מ&quot;ל גלוקוז 5% מתחת לעור. </li><li style=";text-align:right;direction:rtl"> החל העור מטריצה ​​פנטניל טלאים שחרור 12.5 מיקרוגרם / שעה באזור הצוואר מגולח של בעלי חיים על שיכוך כאבים יעיל במהלך 72 שעות. </li><li style=";text-align:right;direction:rtl"> ניהול 250 IU / kg הפרין מולקולרי נמוך יומי תת עורי במשך 2 שבועות. </li></ol> 6. נציג תוצאות: אם ההליך נעשה בצורה נכונה, מפרצת יישאר פטנטים החיות יתאושש בתוך כ - 3 ימים. מניסיוננו ההליכים הדמיה יש לבצע לפחות שבוע לאחר מכן. אחרת החיות ואולי לא תסבול את ההליך יכול להיות אבוד. עבור אמבוליזציה היינו ממליצים להמתין כ 4 שבועות כדי להבטיח משליםendothelialization te של מפרצת neck.Using הטכניקות שהוצגו וניהול המחברים יכלו להגיע patency מפרצת של 85.7% וללא תמותה בסדרה שפורסם לאחרונה שלהם. 14 על מנת לצמצם את שיעור גבוה של סיבוכים במערכת העיכול דיווחה של עד 20%, 12 סיפקנו ניהול ממושך analgetic שיכוך כאבים לאחר הניתוח של 72 שעות לעומת 24 שעות וניהול analgesia.This שגרתי החריפה הביאה שום סיבוכים קיבה. הנהלת קרישה אגרסיבי חשוב להגיע מספיק patency שיעורי מפרצת. זה הולך יחד עם ממצאי גרונוולד et al. מראה את ההשפעות החיוביות של קרישה משולב. 17 בגלל קרישה אגרסיבי הוא חיוני יש איטום יעיל של השקה. עשינו ניסיון טוב מאוד באמצעות דבק הפיברין חדש (Evicel, Ethicon Biosurgery בע&quot;מ, ניו ג&#39;רזי, ארה&quot;ב). בנוסף אנו ממליצים להשתמש ארנבות קטנות (מקסימום 3.5 ק&quot;ג), כמו גם סיבוכים הניסיון שלנו קשורים לעיתים קרובות לנושאים בעלי משקל עודף.

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אין ניגודי אינטרסים הכריז.

החוקרים ממליצים על מתחשב את השלבים הבאים קריטיים מפתח כירורגית על מנת להשיג תוצאות טובות מפרצת פטנט: <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> דיסקציה זהירה של קטע ארוך של המרכז לאמנות עכשווית עזב השקה קל ללא הרס של עצבים וכלי קטן. </li><li style=";text-align:right;direction:rtl">...

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><th> שם מגיב </th><th> חברה </th><th> מספר קטלוגי </th><th> תגובות </th></tr><tr><td> התפרים לרפאות Vicryl4-0 polyfilament </td><td> Ethicon Inc </td><td> J386H </td><td></td></tr><tr><td> Ethilon 10-0 monofil התפרים resorbable הלא </td><td> Ethicon Inc </td><td> 2814 </td><td> נקודת להתחדד מחט </td></tr><tr><td> Evicel Bioglue </td><td> Ethicon Biosurgery בע&quot;מ </td><td> 3901 </td><td></td></tr><tr><td> פנטניל תיקון עורי 12.5 מיקרוגרם / h </td><td></td><td></td><td> כל genericon </td></tr><tr><td> הפרין </td><td></td><td></td><td> כל genericon </td></tr><tr><td> 0.9% Saline </td><td></td><td></td><td> כל genericon </td></tr><tr><td> 4% Papaverin HCl </td><td></td><td></td><td> כל genericon </td></tr><tr><td> Neomycin סולפט 5 מ&quot;ג / מ&quot;ל </td><td></td><td></td><td> כל genericon </td></tr><tr><td> Ketamin 50mg/ml </td><td></td><td></td><td> כל genericon </td></tr><tr><td> Xylazine 20mg/ml </td><td></td><td></td><td> כל genericon </td></tr></tbody></table>

microsurgical venous pouch arterial-bifurcation aneurysms in the rabbit model: technical aspects

עבור מקרע מפרצת המוח האנושי אמבוליזציה endovascular הפכה חלופה שוות ערך גזיר מפרצת. 1 עם זאת, מחקרים קליניים גדולים הוכיחו מאכזב תוצאות לטווח ארוך עם שיעור גבוה פסולה של recanalization מפרצת ו קרע מפרצת מתעכב. 2 כדי להתגבר על בעיות אלה, מחקרים ניסויים בבעלי חיים הם קריטיים לפיתוח של מכשירים endovascular טוב יותר. 3-5 במודלים של בעלי חיים כמה בחולדות, ארנבים, כלבים וחזירים זמינים. 6-8 השוואות של מודלים של בעלי חיים שונים הראו את עליונות של המודל ארנב ביחס hemodynamics ו השוואה של מערכת הקרישה ועלות משתלמת. 9-11 כיס מודל עורקי וורידי הסתעפות בארנבונים נוצר כיס ורידי sutured אל הסתעפות נכון שנוצר באופן מלאכותי של שני עורקי הראש הנפוצים (CCA). היתרון העיקרי של מודל זה הם hemodynamics bifurcational נכון. 12 החסרונות העיקריים הם הדרישות microsurgical גבוה בצופר טכני תחלואה גבוהה ושיעורי תמותה של עד 50%. 13 הגבלות אלה הביאו שימוש תכוף פחות של מודל זה של מפרצת האחרונה שנים. חסרונות אלה ניתן להתגבר עם ניתוחים משופרת שונה peri-וניהול analgetic קרישה לאחר הניתוח. בטכניקות שלנו 14-16 דיווחו במאמר זה להדגים את הטכניקה הזו מותאם ליצירת microsurgical של מפרצת הסתעפות העורקים.

Watch this Scientific Journal Video about Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects at JoVE.com

Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects

טכניקה מותאם ליצירת microsurgical הסתעפות של מפרצת בעורקים מחקה אדם הסתעפות מפרצת מוחית מתואר. כיס ורידי הוא sutured אל הסתעפות נכון שנוצר באופן מלאכותי של שני עורקי הראש המשותף. טכניקות microsurgical הקל ועל קרישה לאחר הניתוח כאבים אגרסיבי להוביל שיעורי תחלואה גבוהים ממוזער patency שיעורי מפרצת.

Microsurgical ורידי עורקי פאוץ-הסתעפות מפרצת במודל הארנב: היבטים טכניים

For ruptured human cerebral aneurysms endovascular embolization has become an equivalent alternative to aneurysm clipping.1 However, large clinical trials ...

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Research

JoVE Journal

Medicine

13.9K Views.  Hospital Rudolfstiftung. טכניקה מותאם ליצירת microsurgical הסתעפות של מפרצת בעורקים מחקה אדם הסתעפות מפרצת מוחית מתואר. כיס ורידי הוא sutured אל הסתעפות נכון שנוצר באופן מלאכותי של שני עורקי הראש המשותף. טכניקות microsurgical הקל ועל קרישה לאחר הניתוח כאבים אגרסיבי להוביל שיעורי תחלואה גבוהים ממוזער pa...

Video: Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects

Technical Aspects of the Mouse Aortocaval Fistula

Technical aspects of creating an arteriovenous fistula in the mouse are discussed. Under general anesthesia, an abdominal incision is made, and the aorta and inferior vena cava (IVC) are exposed. The proximal infrarenal aorta and the distal aorta are dissected for clamp placement and needle puncture, respectively. Special attention is paid to avoid dissection between the aorta and the IVC. After clamping the aorta, a 25 G needle is used to puncture both walls of the aorta into the IVC. The surrounding connective tissue is used for hemostatic compression. Successful creation of the AVF will show pulsatile arterial blood flow in the IVC. Further confirmation of successful AVF can be achieved by post-operative Doppler ultrasound.

The goal is to produce an arteriovenous fistula that is simple and reproducible. This method does not use sutures or glue adhesive. Therefore the samples can be used with the least amount of foreign materials for analysis.

היבטים טכניים של מורסת Aortocaval מאוס

Technical aspects of creating an arteriovenous fistula in the mouse are discussed. Under general anesthesia, an abdominal incision is made, and the aorta ...

The Helsinki Rat Microsurgical Sidewall Aneurysm Model

Experimental saccular aneurysm models are necessary for testing novel surgical and endovascular treatment options and devices before they are introduced into clinical practice. Furthermore, experimental models are needed to elucidate the complex aneurysm biology leading to rupture of saccular aneurysms.
Several different kinds of experimental models for saccular aneurysms have been established in different species. Many of them, however, require special skills, expensive equipment, or special environments, which limits their widespread use. A simple, robust, and inexpensive experimental model is needed as a standardized tool that can be used in a standardized manner in various institutions.
The microsurgical rat abdominal aortic sidewall aneurysm model combines the possibility to study both novel endovascular treatment strategies and the molecular basis of aneurysm biology in a standardized and inexpensive manner. Standardized grafts by means of shape, size, and geometry are harvested from a donor rat&#39;s descending thoracic aorta and then transplanted to a syngenic recipient rat. The aneurysms are sutured end-to-side with continuous or interrupted 9-0 nylon sutures to the infrarenal abdominal aorta.
We present step-by-step procedural instructions, information on necessary equipment, and discuss important anatomical and surgical details for successful microsurgical creation of an abdominal aortic sidewall aneurysm in the rat.

Microsurgical sidewall aneurysms in rats are created by end-to-side anastomosis of an aortic graft to the abdominal aorta. We present step-by-step instructions and discuss anatomical and surgical details for successful experimental saccular aneurysm creation.

הדפנות מפרצת דגם הלסינקי עכברוש Microsurgical

Experimental saccular aneurysm models are necessary for testing novel surgical and endovascular treatment options and devices before they are introduced ...

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and controllable animal models that simulate both conditions are presently uncommon. Therefore, new models are needed in order to mimic human pathophysiological conditions resulting from SAH.
This report describes the technical nuances of a rabbit blood-shunt SAH model that enables control of intracerebral pressure (ICP). An extracorporeal shunt is placed between the arterial system and the subarachnoid space, which enables examiner-independent SAH in a closed cranium. Step-by-step procedural instructions and necessary equipment are described, as well as technical considerations to produce the model with minimal mortality and morbidity. Important details required for successful surgical creation of this robust, simple and consistent ICP-controlled SAH rabbit model are described.

The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures &#8212; subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

דגם ארנב דם מחלף לחקר חריף והמאוחר Sequelae של דימום תת עכבישים: היבטים טכניים

Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and ...

A Murine Model of Arterial Restenosis: Technical Aspects of Femoral Wire Injury

Cardiovascular disease caused by atherosclerosis is the leading cause of death in the developed world. Narrowing of the vessel lumen, due to atherosclerotic plaque development or the rupturing of established plaques, interrupts normal blood flow leading to various morbidities such as myocardial infarction and stroke. In the clinic endovascular procedures such as angioplasty are commonly performed to reopen the lumen. However, these treatments inevitably damage the vessel wall as well as the vascular endothelium, triggering an excessive healing response and the development of a neointimal plaque that extends into the lumen causing vessel restenosis (re-narrowing). Restenosis remains a major cause of failure of endovascular treatments for atherosclerosis. Thus, preclinical animal models of restenosis are vitally important for investigating the pathophysiological mechanisms as well as translational approaches to vascular interventions. Among several murine experimental models, femoral artery wire injury is widely accepted as the most suitable for studies of post-angioplasty restenosis because it closely resembles the angioplasty procedure that injures both endothelium and vessel wall. However, many researchers have difficulty utilizing this model due to its high degree of technical difficulty. This is primarily because a metal wire needs to be inserted into the femoral artery, which is approximately three times thinner than the wire, to generate sufficient injury to induce prominent neointima. Here, we describe the essential surgical details to effectively overcome the major technical difficulties of this model. By following the presented procedures, performing the mouse femoral artery wire injury becomes easier. Once familiarized, the whole procedure can be completed within 20 min.

The mouse femoral artery wire injury model of restenosis is technically challenging. In this protocol we show the key technical details essential for successfully performing wire injury to induce consistent&#160;neointima for studies of restenosis.

דגם Murine של עורקי היצרות: היבטים טכניים של עצם הירך חוט פגיעה

Cardiovascular disease caused by atherosclerosis is the leading cause of death in the developed world. Narrowing of the vessel lumen, due to ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and drugs. It is widely used in many studies on the physiological functions of vascular smooth muscle, the pathogenesis of vascular diseases such as hypertension, and the development of smooth muscle relaxant drugs. The mouse is a widely used model animal with a large pool of disease models and genetically modified strains. We introduced this method to measure the isometric contraction of mouse mesenteric artery in detail. A 1.4-mm segment of mouse mesenteric resistance artery was isolated and mounted on a myograph chamber by passing two steel wires through its lumen. After equilibration and normalization steps, the vessel segment was potentiated by a high-K+ solution twice prior to the contraction assay. As an example of the application of this method in drug development, we measured the relaxant effect of a novel natural substance, neoliensinine, isolated from a Chinese herb, embryos of the lotus seed (Nelumbo nucifera Gaertn.) on mouse mesenteric arteries. The vessel segments mounted on the myograph chamber were stimulated with a high-K+ solution. When the force tension reached a stable sustained phase, cumulative doses of neoliensinine were added to the chamber. We found that neoliensinine had a dose-dependent relaxant effect on smooth muscle contraction, thus suggesting that it bears potential activity against hypertension. In addition, as the vessel segment can survive at least 4 hours after mounting and maintain contractility induced by the high-K+ solution for many times, we suggest that the wire myograph system may be used for the time-consuming process of drug screening.

The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.

Contractility איזומטרי מדידה של עורק מצע המעי העליון העכבר באמצעות חוט Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Transthoracic Echocardiographic Examination in the Rabbit Model

Large animal models such as the rabbit are valuable for translational preclinical research. Rabbits have a similar cardiac electrophysiology compared to that of humans and that of other large animal models such as dogs and pigs. However, the rabbit model has the additional advantage of lower maintenance costs compared to other large animal models. The longitudinal evaluation of cardiac function using echocardiography, when appropriately implemented, is a useful methodology for preclinical assessment of novel therapies for heart failure with reduced ejection fraction (e.g. cardiac regeneration). The correct use of this non-invasive tool requires the implementation of a standardized examination protocol following international guidelines. Here we describe, step by step, a detailed protocol supervised by veterinary cardiologists for performing echocardiography in the rabbit model, and demonstrate how to correctly obtain the different echocardiographic views and imaging planes, as well as the different imaging modes available in a clinical echocardiography system routinely used in human and veterinary patients.

Here we describe, step by step, a detailed protocol for performing echocardiography in the rabbit model. We show how to correctly obtain the different echocardiographic views and imaging planes, as well as the different imaging modes available in a clinical echocardiography system routinely used in human and veterinary patients.

בחינת החזה הקרדיוגרפית במודל הארנב

Large animal models such as the rabbit are valuable for translational preclinical research. Rabbits have a similar cardiac electrophysiology compared to ...

A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure

Although vein grafts have been commonly used as autologous grafts in revascularization surgeries for ischemic diseases, the long-term patency remains poor because of the acceleration of intimal hyperplasia due to the exposure to arterial blood pressure. The present protocol is designed for the establishment of experimental venous intimal hyperplasia by interposing rabbit jugular veins to the ipsilateral carotid arteries. The protocol does not require surgical procedures deep in the body trunk and the extent of the incision is limited, which is less invasive for the animals, allowing long-term observation after implantation. This simple procedure enables researchers to investigate strategies to attenuate the progression of intimal hyperplasia of the implanted vein grafts. Using this protocol, we reported the effects transduction of microRNA-145 (miR-145), which is known to control the phenotype of vascular smooth muscle cells (VSMCs) from the proliferative to the contractile state, into harvested vein grafts. We confirmed the attenuation of intimal hyperplasia of vein grafts by transducing miR-145 before implantation surgery through the phenotype change of the VSMCs. Here we report a less invasive experimental platform to investigate the strategies that can be used to attenuate intimal hyperplasia of vein grafts in revascularization surgeries.

The present protocol aims to experimentally create venous intimal hyperplasia by subjecting veins to arterial blood pressure for developing strategies to attenuate venous intimal hyperplasia following revascularization surgery using vein grafts.

ורידי ארנב מודל האינטרפוזיציה מחקה ניתוח מחדש באמצעות שתלי וריד כדי להעריך את היפרפלזיה באמצעות לחץ דם עורקי

Although vein grafts have been commonly used as autologous grafts in revascularization surgeries for ischemic diseases, the long-term patency remains poor ...

Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit

Preclinical animal models with hemodynamic, morphologic, and histologic characteristics close to human intracranial aneurysms play a key role in the understanding of the pathophysiological processes and the development and testing of new therapeutic strategies. This study aims to describe a new rabbit aneurysm model that allows the creation of two elastase-digested saccular aneurysms with different hemodynamic conditions within the same animal.
Five female New Zealand white rabbits with a mean weight of 4.0 (&#177; 0.3) kg and mean age of 25 (&#177;5) weeks underwent microsurgical stump and bifurcation aneurysm creation. One aneurysm (stump) was created by right common carotid artery (CCA) exposure at its origin at the brachiocephalic trunk. A temporary clip was applied at the CCA origin and another, 2 cm above. This segment was treated with a local injection of 100 U of elastase for 20 min. A second aneurysm (bifurcation) was created by suturing an elastase-treated arterial pouch into the end-to-side anastomosis of the right CCA to left CCA. Patency was controlled by fluorescence angiography immediately after creation.
The average duration of surgery was 221 min. The creation of two aneurysms in the same animal was successful in all rabbits without complication. All aneurysms were patent immediately after surgery except for one bifurcation aneurysm, which showed an extreme tissue reaction due to elastase incubation and an immediate intraluminal thrombosis. No mortality was observed during surgery and up to one-month follow-up. Morbidity was limited to a transient vestibular syndrome (one rabbit), which recovered spontaneously within one day.
Demonstrated here for the first time is the feasibility of creating a two-aneurysm rabbit model with stump and bifurcation hemodynamic characteristics and highly degenerated wall conditions. This model allows the study of the natural course and potential treatment strategies on the basis of aneurysm biology under different flow conditions.

This protocol describes the steps for the creation of a rabbit model with two elastase-digested aneurysms with different hemodynamics (stump and bifurcation constellation). This allows the testing of novel endovascular devices in aneurysms with different angioarchitecture and hemodynamic conditions within a single animal.

יצירת שתי מפרצות מעוכלות באלסטאז עם המודינמיקה שונה בארנב אחד

Preclinical animal models with hemodynamic, morphologic, and histologic characteristics close to human intracranial aneurysms play a key role in the ...

Isolated Lung Perfusion System in the Rabbit Model

The isolated lung perfusion system has been widely used in pulmonary research, contributing to elucidate the lungs&#39; inner workings, both micro and macroscopically. This technique is useful in the characterization of pulmonary physiology and pathology by measuring metabolic activities and respiratory functions, including interactions between circulatory substances and the effects of inhaled or perfused substances, as in drug testing. While in vitro methods involve the slicing and culturing of tissues, the isolated ex vivo lung perfusion system allows to work with a complete functional organ making possible the study of a continuous physiological function while recreating ventilation and perfusion. However, it should be noted that the effects of the absence of central innervation and lymphatic drainage still have to be fully assessed. This protocol aims to describe the assembly of the isolated lung apparatus, followed by the surgical extraction and cannulation of lungs and heart from experimental lab animals, as well as to display the perfusion technique and signal processing of data. The average viability of the isolated lung ranges between 5-8 h; during this period, the pulmonary capillary permeability increases, causing edema and lung injury. The functionality of preserved pulmonary tissue is measured by the capillary filtration coefficient (Kfc), used to determine the extent of pulmonary edema through time.

The isolated rabbit lung preparation is a gold standard tool in pulmonary research. This publication aims to describe the technique as developed for the study of physiological and pathological mechanisms involved in airway reactivity, lung preservation, and preclinical research in lung transplantation and pulmonary edema.

מערכת זלוף ריאות מבודדת במודל הארנב

The isolated lung perfusion system has been widely used in pulmonary research, contributing to elucidate the lungs&#39; inner workings, both micro and ...

A Rabbit Aortic Valve Stenosis Model Induced by Direct Balloon Injury

Animal models are emerging as an important tool to understand the pathologic mechanisms underlying aortic valve stenosis (AVS) because of the lack of access to reliable sources of diseased human aortic valves. Among the various animal models, AVS rabbit models are one of the most commonly used in large animal studies. However, traditional AVS rabbit models require a long-term period of dietary supplementation and genetic manipulation to induce significant stenosis in the aortic valve, limiting their use in experimental studies. To address these limitations, a new AVS rabbit model is proposed, in which stenosis is induced by a direct balloon injury to the aortic valve. The present protocol describes a successful technique for inducing AVS in New Zealand white (NZW) rabbits, with step-by-step procedures for the preparation, the surgical procedure, and the post-operative care. This simple and reproducible model offers a promising approach for studying the initiation and progression of AVS and provides a valuable tool for investigating the underlying pathological mechanisms of the disease.

An appropriate animal model is needed to understand the pathologic mechanisms underlying aortic valve stenosis (AVS) and to evaluate the efficacy of therapeutic interventions. The present protocol describes a new procedure for developing the AVS rabbit model via a direct balloon injury in vivo.

מודל היצרות של מסתם אבי העורקים של ארנב הנגרם על ידי פגיעה ישירה בבלון

Animal models are emerging as an important tool to understand the pathologic mechanisms underlying aortic valve stenosis (AVS) because of the lack of ...