We thank the Duke Physics Shop, especially Phil Lewis, for helping us build the behavioral apparatus and attachments and creating the drawing. This work is funded by the National Institutes of Health under award number R01GM100027.

1. הכנת זבובים להיות assayed <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> תרבות עפה על מולסה הסטנדרטי / מדיה קמח תירס בבית החממה נקבעה על 25 מעלות צלזיוס ו -65% לחות. תשמור על עצמך כדי לא לגדוש הצלוחיות. לדוגמא, לשים 8 נקבות ו 6 זכרים לתוך בקבוקון מזון צר. הערה: &quot;בקבוקון מזון צר&quot; משמש כאן יש בקוטר פנימי של 2.3 ס&quot;מ. אנחנו בדרך כלל לוותר על 10 מ&quot;ל של אוכל לטוס לתוך בקבוקון אחד. המתכון אוכל לטוס השתמשנו מתואר כאן: http://flystocks.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.html. </li><li style=";text-align:right;direction:rtl"> 2 - 3 ימים לאחר eclosion הנקבות, להכין צלוחיות עם רסק שמרים טריים כפי שמוצג באיור 1E. הפוך רסק שמרים טריים ידי ערבוב 6 גרם של שמרים פעילים עם 10 מ&quot;ל של חומצה proprionic 0.5%. להשתמש במרית להחיל את הרסק שמרים על הדפנות של צלוחיות המזון. אסוף 30 - 35 נקבות eclosed יחד עם 20 - 25 זכרים לתוך צלוחיות. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> באופן כללי, לאסוף זכרים מאותו הבקבוקונים שבו הנקבות נאספות(כדי לחסוך זמן ומאמץ). עם זאת, אם קיים חשש לגבי הפוריות של גברים, להשתמש זכרי WT במקום. כמו כן, רסק שמרים חשוב מגרה ייצור ביצים ולכן להכין אותו טרי מדי יום. </li></ol></li><li style=";text-align:right;direction:rtl"> לשמור על נקבות / זכרים שנאספו על 25 מעלות צלזיוס, 65% לחות, אלא אם הזבובים הם טמפרטורה רגישה. </li><li style=";text-align:right;direction:rtl"> לאחר ~ 4 - 5 ימים, לבדוק את הבקבוקונים כדי לראות אם נקבות מוכנות לניסויי הטלה. הם מוכנים כאשר השטח של המדיום מזון רטוב מן הזחלים נבירה פעיל בתחום המזון (ראה איור 1E - F). הערה: נקבות בדרך כלל לעכב ביצים שתחזינה השטח האוכל הופך להיות רטוב בהם זחלים. צעד זה מבטיח כי נקבות תהיינה מוכנות להטיל ביצים רבות כאשר הם נתקלים מצעים עם מרקם רצוי (למשל., ~ 1% agarose). </li></ol> 2. בנייה קאמרית, הרכבה, התקנת Assay <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> יש חנות מכונית לבנות תאי אקריליק הטלה (אלחוטיאיור 1 א - ד). ציור ההנדסה של חלקים שונים הוצג משלים איור 1 -. 3 תמונות באיכות גבוהה ניתן למצוא גם כאן (http://www.rebeccayang.org/pdf/chamber%20design.pdf). </li><li style=";text-align:right;direction:rtl"> הכנס יריעות פלסטיק לתוך טעינה (למעלה) חתיכה של החדר כפי שמוצג באיור 1C. זה משמש את המשטח התחתון בזמן טעינת זבובים לתוך זירות ההטלה הפרט. </li><li style=";text-align:right;direction:rtl"> להרדים נקבות על כרית CO 2 ומעלה אותם בנפרד לתוך אחד ממרכיבי הטלה. אפשר ~ 30 דקות לזבובים להתאושש CO 2 ולהפוך הסתגלו לסביבה החדשה. </li><li style=";text-align:right;direction:rtl"> הכן את מצעי agarose. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> לנוחיותכם, להחזיק בקבוק התערובת של העוגיות של agarose 1% נמס בתוך אמבט מים 55 מעלות צלזיוס. </li><li style=";text-align:right;direction:rtl"> מוסיפים את הכמות הרצויה של תמיסת סוכרוז המניות (2 מ &#39;) לתוך צינור חרוטי 50 מ&quot;ל ו לערבב אותו עם כמות מתאימה של agarose. לדוגמה, כדי להכין את המצע סוכרוז 150 מ&quot;מ, PLAce 750 μl של 2 M תמיסת סוכרוז לתוך הצינור ואז למלא את הצינור עם agarose אל 10 מ&quot;ל סימן. </li><li style=";text-align:right;direction:rtl"> מכין את המצע הרגיל באותו אופן, אלא להוסיף מים מזוקקים במקום תמיסת סוכרוז. הערה: הריכוז הסופי של agarose בפרוטוקול זה הוא מעט פחות מ -1%. מניסיוננו, הריכוז המדויק של agarose לא משנה כל עוד היא נשלטת להיות בתוך ~ 0.9 - 1.1%, וכי שני המצעים הם מאותו הריכוז agarose. </li></ol></li><li style=";text-align:right;direction:rtl"> קח את המצע (למטה) חתיכה של החדר ואת פיפטה 1,000 μl של המצע agarose לתוך כל שוקת כפי שניתן לראות באיור 1D. </li><li style=";text-align:right;direction:rtl"> אפשר agarose כדי לחזק עבור ~ 30 דקות. </li><li style=";text-align:right;direction:rtl"> לאחר המצעים וזבובי agarose מוכנים, להרכיב את כל שלוש החתיכות של חדר ההטלה ולאחר מכן להוציא את יריעות הפלסטיק. </li><li style=";text-align:right;direction:rtl"> מניח את התאים באינקובטורים זבוב. הערה: אורך של ניסויי הטלה יכול להשתנות בהתאם expeהצרכים rimental. אנחנו בדרך כלל להפעיל את הניסוי O / N (14 - 16 שעות). כמו כן, אין השפעה משמעותית של עיתוי יממה על העדפות הטלה נצפתה. </li><li style=";text-align:right;direction:rtl"> להרדים נשים על ידי הזרקת CO 2 לתוך התא. לפרק את התא, לבטל את הזבובים הרדימו לתוך מתי זבוב (כלומר., מקפה ריק יכול מלא מעט שמן תירס). צלמו תמונות של התוצאות עבור ניהול ספרים (ראה איור 2). </li><li style=";text-align:right;direction:rtl"> ספירת הביצים ידנית ולחשב מדדי העדפה לניתוח. חישוב מדד העדפה לגבי (N א - N ב) / (N א ב + N) שבו N ו- B N לייצג מספר הביצים באתר א ב האתר לעומת, בהתאמה. </li></ol>

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The authors have nothing to disclose.

The chambers and protocols described here have several improvements over previous egg-laying assays. First, they increase the throughput of assaying preferences of single animals significantly. Each chamber can assay 30 single females and it takes less than an hour to set up. Second, they increase the consistency of the egg-laying preferences over previous methods. The standardization of the dimensions of the arena, size of the egg-laying substrates, and distance between substrates makes it easier to compare the results ...

Drosophila melanogaster is a powerful genetic model organism to study the neural basis of behaviors. The rapid developments of genetic tools to manipulate neurons in a targeted manner and the emergence of sophisticated behavioral analysis tools have significantly improved our ability to dissect the circuit mechanisms that underlie the sensory-motor transformation processes of several innate and learned behaviors1-3.
Drosophila egg-laying is a suitable model to study the neural basis of simple decision making processes. In particular, Drosophila females have been shown to possess the ability to compare and rank their options before "committing" to depositing an egg onto a given option4-8. For example, when given only a plain (sucrose-free) substrate or only a sucrose-containing substrate, females readily accept either option for egg-laying. However, when presented with both options, females robust reject the sucrose substrate in some contexts7,9,10. Relatively little is known about the neural mechanisms that allow females to "choose the greater of two goods", however. A major obstacle has been the lack of an efficient method to assay egg-laying preferences such that one can use a systematic genetic screening approach to study this problem.
In this report, we describe the protocol we developed that allows egg-laying preferences of females to be assayed at single-animal resolution and with substantially improved throughput and consistency over previous methods. Specifically, we provide the blueprints for constructing the chambers we designed, the protocol for preparing the females so that each is primed to lay many eggs, and the protocol for using the chambers.

<table><tbody><tr><td>UltraPure Agarose&amp;nbsp;</td><td>Invitrogen</td><td>16500-500</td><td></td></tr><tr><td>Sucrose</td><td>Sigma</td><td>S0389</td><td></td></tr><tr><td>Water bath&amp;nbsp;</td><td>Fisher</td><td>15-462-6Q</td><td></td></tr><tr><td>LifeCam Cinema webcam</td><td>Microsoft</td><td>H5D-00013</td><td></td></tr><tr><td>Red LEDs</td><td>Cree</td><td>C503B-RAN-CA0B0AA1</td><td></td></tr><tr><td>Egg-laying chambers</td><td>Custom Built</td><td></td><td></td></tr><tr><td>Camera holders</td><td>Custom Built</td><td></td><td></td></tr><tr><td>LED holders</td><td>Custom Built</td><td></td><td></td></tr><tr><td>Fly vials (narrow)</td><td>Genesee</td><td>32-116BC</td><td></td></tr></tbody></table>

high throughput assay to examine egg-laying preferences of individual drosophila melanogaster

לאחרונה, העדפת הטלה של תסיסנית התפתחה כמודל צייתן גנטי ללמוד את הבסיס העצבי של תהליכי קבלת החלטות פשוטים. בעת בחירת אתרים מטילים את ביציהן, נקבות זבוב מסוגל בדירוג האטרקטיביות היחסית של האופציות שלהם ובחירת &quot;יותר משני מוצרים.&quot; עם זאת, מבחני ההעדפה ביותר ההטלה אינם מעשיים אם רוצה לנקוט בגישת סריקה גנטית שיטתית לחיפוש הבסיס המעגל שבבסיס תהליך קבלת החלטות פשוט זו, כפי שהן מבוססות על אוכלוסייה ומייגעת להקים. כדי להגדיל את התפוקה של הלימוד של העדפות הטלה של נקבות אחת, פתחנו תאי מנהג שכל יכול העדפות assay בו זמנית הטלה של עד שלושים פרט עפו כמו גם פרוטוקול המבטיח לכל נקבה יש שיעור גבוה הטלה (כך העדפתם היא להבחין בקלות ועוד משכנע). הגישה שלנו היא פשוטה לביצועומייצר תוצאות מאוד עקביות. בנוסף, החדרים האלה יכולים להיות מצוידים עם קבצים מצורפים שונים כדי לאפשר ווידאו הקלטת חי ההטלה, וכדי לספק אור ללימודי optogenetics. מאמר זה מספק את השרטוטים בודים תאים אלה ואת הליך הכנת הזבובים להיות assayed בתאים אלה.

תאי ההטלה מורכבים מכמה חתיכות: חתיכת מצע (למטה), מחיצה (באמצע) חתיכה, חתיכת טעינה (למעלה), ו -2 דלתות זזות (איור 1 א - ד). יצירות אלה משמשות זבובי התקנה באופן עצמאיים מצעים לפני ניסויי הטלה. איור 1F מראה כיצד בקבוקונים צריכים להיראות כאשר נ...

Watch this Scientific Journal Video about High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster at JoVE.com

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Assay תפוקה גבוהה לבחינת העדפות הטלה של פרט<em&gt; תסיסנית</em

This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. ...

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High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

7.9K Views.  Duke University. This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Video: High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Methods to Assay Drosophila Behavior

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular disease and various neurological diseases1. We have optimized simple and robust behavioral assays for determining larval locomotion, adult climbing ability (RING assay), and courtship behaviors of Drosophila. These behavioral assays are widely applicable for studying the role of genetic and environmental factors on fly behavior. Larval crawling ability can be reliably used for determining early stage changes in the crawling abilities of Drosophila larvae and also for examining effect of drugs or human disease genes (in transgenic flies) on their locomotion. The larval crawling assay becomes more applicable if expression or abolition of a gene causes lethality in pupal or adult stages, as these flies do not survive to adulthood where they otherwise could be assessed. This basic assay can also be used in conjunction with bright light or stress to examine additional behavioral responses in Drosophila larvae. Courtship behavior has been widely used to investigate genetic basis of sexual behavior, and can also be used to examine activity and coordination, as well as learning and memory. Drosophila courtship behavior involves the exchange of various sensory stimuli including visual, auditory, and chemosensory signals between males and females that lead to a complex series of well characterized motor behaviors culminating in successful copulation. Traditional adult climbing assays (negative geotaxis) are tedious, labor intensive, and time consuming, with significant variation between different trials2-4. The rapid iterative negative geotaxis (RING) assay5 has many advantages over more widely employed protocols, providing a reproducible, sensitive, and high throughput approach to quantify adult locomotor and negative geotaxis behaviors. In the RING assay, several genotypes or drug treatments can be tested simultaneously using large number of animals, with the high-throughput approach making it more amenable for screening experiments.

Methods to Assay Drosophila Behavior

Drosophila melanogaster is a genetically and behaviorally tractable model system that has been used to understand the molecular and cellular basis of many important biological processes for over a century 1. Drosophila has been well exploited to gain insights into the genetic basis of fly behavior.

שיטות Assay תסיסנית התנהגות

Drosophila melanogaster, the fruit fly, has been used to study molecular mechanisms of a wide range of human diseases such as cancer, cardiovascular ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer basic questions on how light information is processed and shared between rapid and circadian behaviors. Drosophila larvae display a stereotypical avoidance behavior when exposed to light. To investigate light dependent behaviors comparably simple light-dark preference tests can be applied. In vertebrates and arthropods the neural pathways involved in sensing and processing visual inputs partially overlap with those processing photic circadian information. The fascinating question of how the light sensing system and the circadian system interact to keep behavioral outputs coordinated remains largely unexplored. Drosophila is an impacting biological model to approach these questions, due to a small number of neurons in the brain and the availability of genetic tools for neuronal manipulation. The presented light-dark preference assay allows the investigation of a range of visual behaviors including circadian control of phototaxis.

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Here we describe a light-dark preference test for Drosophila larva. This assay provides information about innate and circadian regulation of light sensing and processing photobehavior.

Assay העדפות אור לחקר Photobehavior מוסדר מולדת ויממה ב<em&gt; דרוזופילה</em&gt; זחלים

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

A Single-fly Assay for Foraging Behavior in Drosophila

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster. In a light-tight box illuminated by red light that is invisible to fruit flies, a camera linked to custom data acquisition software monitors the position of six flies simultaneously. Each fly is confined to walk in individual arenas containing a food odor at the center. The testing arenas rest on a porous floor that functions to prevent odor accumulation. Latency to locate the odor source, a metric that reflects olfactory sensitivity under different physiological states, is determined by software analysis. Here, we discuss the critical mechanics of running this behavioral paradigm and cover specific issues regarding fly loading, odor contamination, assay temperature, data quality, and statistical analysis.

A Single-fly Assay for Foraging Behavior in Drosophila

In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.

יחיד לטוס Assay עבור התנהגות שיחור מזון ב<em&gt; דרוזופילה</em

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video ...

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous system and protects neural tissue from toxins and pathogens. Defects in the formation of this barrier have been correlated with neuropathies, and the breakdown of this barrier has been observed in many neurodegenerative diseases. Therefore, it is critical to identify the genes that regulate the formation and maintenance of the blood-brain barrier to identify potential therapeutic targets. In order to understand the exact roles these genes play in neural development, it is necessary to assay the effects of altered gene expression on the integrity of the blood-brain barrier. Many of the molecules that function in the establishment of the blood-brain barrier have been found to be conserved across eukaryotic species, including the fruit fly, Drosophila melanogaster. Fruit flies have proven to be an excellent model system for examining the molecular mechanisms regulating nervous system development and function. This protocol describes a step-by-step procedure to assay for blood-brain barrier integrity during the embryonic and larval stages of D. melanogaster development.

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.

שיטת שלמות המוח בדם בדרוזופילה מלאנוסטר

Proper nervous system development includes the formation of the blood-brain barrier, the diffusion barrier that tightly regulates access to the nervous ...

Developmental Biology

Measuring and Altering Mating Drive in Male Drosophila melanogaster

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, reductionist, and scalable system for in-depth investigation of motivation using the mating drive of male Drosophila&#160;melanogaster (Drosophila), the methods for which we detail here. The behavioral paradigm centers on the finding that male mating drive decreases alongside fertility over the course of repeated copulations and recovers over ~3 d. In this system, the powerful neurogenetic tools available in the fly converge with the genetic accessibility and putative wiring diagram available for sexual behavior. This convergence allows rapid isolation and interrogation of small neuronal populations with specific motivational functions. Here we detail the design and execution of the satiety assay that is used to measure and alter courtship motivation in the male fly. Using this assay, we also demonstrate that low male mating drive can be overcome by stimulating dopaminergic neurons. The satiety assay is simple, affordable, and robust to influences of genetic background. We expect the satiety assay to generate many new insights into the neurobiology of motivational states.

Measuring and Altering Mating Drive in Male Drosophila melanogaster

This article describes a behavioral assay that uses male mating drive in Drosophila melanogaster to study motivation. Using this method, researchers can utilize advanced fly neurogenetic techniques to uncover the genetic, molecular, and cellular mechanisms that underlie this motivation.

מדידת כונן מזווג סירוס ועיקור במאלה<em&gt; תסיסנית</em

Despite decades of investigation, the neuronal and molecular bases of motivational states remain mysterious. We have recently developed a novel, ...

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Many animals, including the fruit fly, Drosophila melanogaster, are capable of discriminating minute differences in&#160;environmental temperature, which enables them to seek out their preferred thermal landscape. To define the temperature preferences of larvae over a defined linear range, we developed an assay using a temperature gradient. To establish a single-directional gradient, two aluminum blocks are connected to independent water baths, each of which controls the temperature of individual blocks. The two blocks set the lower and upper limits of the gradient. The temperature gradient is established by placing an agarose-coated aluminum plate over the two water-controlled blocks so that the plate spans the distance between them. The ends of the aluminum plate that is set on the top of the water blocks defines the minimum and maximum temperatures, and the regions in-between the two blocks form a linear temperature gradient. The gradient assay can be applied to&#160;larvae of different ages and can be used to identify mutants that exhibit phenotypes, such as those with mutations affecting genes encoding TRP channels and opsins, which are required for temperature discrimination.

A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae

Here, we present a protocol to determine the preferred environmental temperature of Drosophila larvae using a continuous thermal gradient.

וזמינותו שינוי טמפרטורה כדי לקבוע העדפות תרמי של הזחלים דרוזופילה

Many animals, including the fruit fly, Drosophila melanogaster, are capable of discriminating minute differences in&#160;environmental temperature, which ...

A Rapid Food-Preference Assay in Drosophila

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate their food environment. The fruit fly, Drosophila melanogaster, is a genetically tractable model organism that is widely used to decipher the molecular, cellular, and neural underpinnings of food preference. To analyze fly food preference, a robust feeding method is needed. Described here is a two-choice feeding assay, which is rigorous, cost-saving, and fast. The assay is Petri-dish-based and involves the addition of two different foods supplemented with blue or red dye to the two halves of the dish. Then, ~70 prestarved, 2-4-day-old flies are placed in the dish and&#160;allowed to choose between blue and red foods in the dark for about 90 min. Examination of the abdomen of each fly is followed by the calculation of the preference index. In contrast to multiwell plates, each Petri dish takes only ~20 s to fill and saves time and effort. This feeding assay can be employed to quickly determine whether flies like or dislike a particular food.

A Rapid Food-Preference Assay in Drosophila

We present a protocol for a two-choice feeding assay for flies. This feeding assay is fast and easy to run and is suitable not only for small-scale laboratory research, but also for high-throughput behavioral screens in flies.

מבחני העדפת מזון מהירה בדרוסופילה

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate ...

Fly Climbing Assay: A Method to Test General Locomotion in Drosophila melanogaster

Source: Gevedon, O., et al. <a href="https://www.jove.com/video/59720/in-vivo-forward-genetic-screen-to-identify-novel-neuroprotective">In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster</a>. J. Vis. Exp. (2019).
This video describes a simple method to quantify fly locomotion and shows the climbing assay performed.&#160; The fly climbing assay is a general test of motor function which relies on a negative geotaxis response.

בדיקת טיפוס זבובים: שיטה לבדיקת תנועה כללית בדרוזופילה מלנוגסטר

Source: Gevedon, O., et al. In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster. J. Vis. Exp. (2019).
This ...

JoVE Encyclopedia of Experiments

Biology

Drosophila melanogaster (fruit fly)

Adult

Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans

Source: Lee, S. Y. and Kang, K. <a href="https://www.jove.com/video/60419/measuring-effects-bacteria-chemicals-on-intestinal-permeability">Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans</a>.&#160;J. Vis. Exp. (2017).
C. elegans egg-laying behavior can provide important information in developmental, reproductive, and anatomical studies.&#160; This video introduces the important steps of an egg laying assay and shows a sample experiment in which a drug is tested for reproductive toxicity.

בדיקת הטלת ביצים: שיטה לכימות התנהגות הטלת הביצים של C. elegans

Source: Lee, S. Y. and Kang, K. Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans.&#160;J. Vis. Exp. 
...

Caenorhabditis elegans (worm)

Basic Methods

Island Assay: A High-Throughput Method to Evaluate Drosophila Locomotor Behavior

Source: Eidhof, I., et al. <a href="https://www.jove.com/video/55892/" target="_blank">High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay</a>. J. Vis. Exp. (2017).
This video describes the island assay, a behavioral method used to study Drosophila locomotion. The sample protocol features a setup for the assay compatible with semi-automated image analysis.

בדיקת אי: שיטה בעלת תפוקה גבוהה להערכת התנהגות לוקומוטורית של דרוזופילה

Source: Eidhof, I., et al. High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay. J. Vis. Exp. (2017).
This video describes the ...

Assay תפוקה גבוהה לבחינת העדפות הטלה של פרט

Summary

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