Fukushima Medical University

4 ARTICLES PUBLISHED IN JoVE

Behavior

Recording Horizontal Saccade Performances Accurately in Neurological Patients Using Electro-oculogram

Yasuo Terao¹, Hideki Fukuda², Yusuke Sugiyama³, Satomi Inomata-Terada¹, Shin-ichi Tokushige⁴, Masashi Hamada³, Yoshikazu Ugawa⁵

¹Department of Cell Physiology, Kyorin University, ²Segawa Memorial Neurological Clinic for Children, ³Department of Neurology, University of Tokyo, ⁴Department of Neurology, Kyorin University, ⁵Department of Neurology, Fukushima Medical University

The article describes a practical method for recording horizontal eye movements with high accuracy by electro-oculogram in neurological patients, using a cup Ag-AgCl electrode with a wide plastic fringe. Stable measurement requires proper selection and fixation of electrodes, taking sufficient time for light adaptation to occur, and re-calibration as needed.

Medicine

Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy

Eriko Katsuta¹, Masanori Oshi¹, Omar M. Rashid^2,3,4,5, Kazuaki Takabe^1,6,7,8,9,10

¹Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, ²Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, ³Department of Surgery, Massachusetts General Hospital, ⁴Department of Surgery, University of Miami Miller School of Medicine, ⁵Department of Surgery, Nova Southeastern University School of Medicine, ⁶Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, ⁷Department of Breast Surgery and Oncology, Tokyo Medical University, ⁸Department of Surgery, Yokohama City University, ⁹Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, ¹⁰Department of Surgery, Fukushima Medical University

We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.

Developmental Biology

Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

Yuji Watanabe¹, Chie Sakuma¹, Hiroyuki Yaginuma¹

¹Department of Neuroanatomy and Embryology, School of Medicine, Fukushima Medical University

We describe the methods for fluorescent labeling of tangentially migrating cells by electroporation, and for time-lapse imaging of the labeled cell movement in a flat-mount culture in order to visualize migrating cell behavior in the developing chick optic tectum.

Cancer Research

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids

Arisa Higa¹, Nobuhiko Takahashi^2,3, Gen Hiyama², Hirosumi Tamura², Hirotaka Hoshi², Kenju Shimomura³, Shinya Watanabe², Motoki Takagi²

¹FUJIFILM Wako Bio Solutions Corporation, ²Medical-Industrial Translational Research Center, Fukushima Medical University, ³Department of Bioregulation and Pharmacological Medicine, Fukushima Medical University

A highly accurate in vitro high-throughput assay system was developed to evaluate anticancer drugs using patient-derived tumor organoids (PDOs), similar to cancer tissues but are unsuitable for in vitro high-throughput assay systems with 96-well and 384-well plates.