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Fukushima Medical University

4 ARTICLES PUBLISHED IN JoVE

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Behavior

Recording Horizontal Saccade Performances Accurately in Neurological Patients Using Electro-oculogram
Yasuo Terao 1, Hideki Fukuda 2, Yusuke Sugiyama 3, Satomi Inomata-Terada 1, Shin-ichi Tokushige 4, Masashi Hamada 3, Yoshikazu Ugawa 5
1Department of Cell Physiology, Kyorin University, 2Segawa Memorial Neurological Clinic for Children, 3Department of Neurology, University of Tokyo, 4Department of Neurology, Kyorin University, 5Department of Neurology, Fukushima Medical University

The article describes a practical method for recording horizontal eye movements with high accuracy by electro-oculogram in neurological patients, using a cup Ag-AgCl electrode with a wide plastic fringe. Stable measurement requires proper selection and fixation of electrodes, taking sufficient time for light adaptation to occur, and re-calibration as needed.

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Medicine

Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy
Eriko Katsuta 1, Masanori Oshi 1, Omar M. Rashid 2,3,4,5, Kazuaki Takabe 1,6,7,8,9,10
1Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, 2Holy Cross Hospital Michael and Dianne Bienes Comprehensive Cancer Center, 3Department of Surgery, Massachusetts General Hospital, 4Department of Surgery, University of Miami Miller School of Medicine, 5Department of Surgery, Nova Southeastern University School of Medicine, 6Department of Surgery, University at Buffalo Jacobs School of Medicine and Biomedical Sciences, 7Department of Breast Surgery and Oncology, Tokyo Medical University, 8Department of Surgery, Yokohama City University, 9Department of Surgery, Niigata University Graduate School of Medical and Dental Sciences, 10Department of Surgery, Fukushima Medical University

We introduce a murine orthotopic breast cancer model and radical mastectomy model with bioluminescence technology to quantify the tumor burden to mimic human breast cancer progression.

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Developmental Biology

Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum
Yuji Watanabe 1, Chie Sakuma 1, Hiroyuki Yaginuma 1
1Department of Neuroanatomy and Embryology, School of Medicine, Fukushima Medical University

We describe the methods for fluorescent labeling of tangentially migrating cells by electroporation, and for time-lapse imaging of the labeled cell movement in a flat-mount culture in order to visualize migrating cell behavior in the developing chick optic tectum.

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Cancer Research

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids
Arisa Higa 1, Nobuhiko Takahashi 2,3, Gen Hiyama 2, Hirosumi Tamura 2, Hirotaka Hoshi 2, Kenju Shimomura 3, Shinya Watanabe 2, Motoki Takagi 2
1FUJIFILM Wako Bio Solutions Corporation, 2Medical-Industrial Translational Research Center, Fukushima Medical University, 3Department of Bioregulation and Pharmacological Medicine, Fukushima Medical University

A highly accurate in vitro high-throughput assay system was developed to evaluate anticancer drugs using patient-derived tumor organoids (PDOs), similar to cancer tissues but are unsuitable for in vitro high-throughput assay systems with 96-well and 384-well plates.

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