Douglas Mental Health University Institute

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy

Marie-Eve Tremblay¹, Mustapha Riad², Ania Majewska¹

¹Department of Neurobiology and Anatomy, University of Rochester, ²Douglas Mental Health University Institute

We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.

Neuroscience

How to Find Effects of Stimulus Processing on Event Related Brain Potentials of Close Others when Hyperscanning Partners

Amanda Tardif^1,3, Ashley Chau-Morris^1,2, Zi Yue Wang¹, Ehime Takahara¹, Tim Hadjis^1,2, Jean Debruille¹, J. Bruno Debruille^1,2,3

¹Douglas Mental Health University Institute, ²Department of Psychiatry, McGill University, ³Department of Neurology and Neurosurgery, McGill University

This protocol describes key steps involved in assessing the sensitivity of the brain of one person to the stimulus processing of a close other by selecting pairs of partners, recording their electroencephalogram (EEG) simultaneously and computing their event-related brain potentials (ERPs).