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Preparation of Mouse Brain Tissue for Immunoelectron Microscopy

DOI :

10.3791/2021-v

July 20th, 2010

July 20th, 2010

36,126 Views

1Department of Neurobiology and Anatomy, University of Rochester, 2Douglas Mental Health University Institute

We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.

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Mouse Brain Tissue

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