Visualization of Organelles In Situ by Cryo-STEM TomographyPeter Kirchweger 1, Debakshi Mullick 1, Sharon Grayer Wolf 2, Michael Elbaum 1
1Department of Chemical and Biological Physics, Weizmann Institute of Sciences, 2Department of Chemical Research Support, Weizmann Institute of Sciences
Cryo-STEM tomography provides a means to visualize organelles of intact cells without embedding, sectioning, or other invasive preparations. The 3D resolution obtained is currently in the range of a few nanometers, with a field of view of several micrometers and an accessible thickness in the order of 1 µm.
