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Visualization of Organelles In Situ by Cryo-STEM Tomography

DOI :

10.3791/65052-v

8:37 min

June 23rd, 2023

June 23rd, 2023

1,009 Views

1Department of Chemical and Biological Physics, Weizmann Institute of Sciences, 2Department of Chemical Research Support, Weizmann Institute of Sciences

Cryo-STEM tomography provides a means to visualize organelles of intact cells without embedding, sectioning, or other invasive preparations. The 3D resolution obtained is currently in the range of a few nanometers, with a field of view of several micrometers and an accessible thickness in the order of 1 µm.

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STEM Tomography

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