Detection of Abnormal Prion Protein by ImmunohistochemistryLeonor Orge 1,2, Maria de Lurdes Pinto 1, Paula Cristovão 2, Paula Mendonça 2, Paulo Carvalho 2, Carla Lima 3, Helena Santos 2, Anabela Alves 1, Fernanda Seixas 1, Isabel Pires 1, Adelina Gama 1, Maria dos Anjos Pires 1
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão
Immunolabeling abnormal prion protein using immunohistochemistry protocols requires specific sample and anti-PrP antibody preparation methodologies. The present protocol describes the key steps in epitope demasking to ensure proper PrP immunolabeling and to minimize non-specific background staining. Also, this approach considers biosafety measures when conducting immunohistochemistry studies with the prion-infected tissues.
