Detection of Abnormal Prion Protein by Immunohistochemistry
2.7K Views
•
06:38 min
•
May 5th, 2023
DOI :
May 5th, 2023
•Leonor Orge1,2, Maria de Lurdes Pinto1, Paula Cristovão2, Paula Mendonça2, Paulo Carvalho2, Carla Lima3, Helena Santos2, Anabela Alves1, Fernanda Seixas1, Isabel Pires1, Adelina Gama1, Maria dos Anjos Pires1
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão
Explore More Videos
Chapters in this video
0:04
Introduction
0:48
Deparaffinization and Rehydration
1:39
Epitope Retrieval
2:31
Inactivation of Endogenous Peroxidase and Immunodetection
4:59
Results: Epitope Demasking for Proper PrP Immunolabeling and Minimizing Non-Specific Background Staining
6:00
Conclusion
Related Videos
09:22
Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
11.4K Views
06:02
Detection of Neuritic Plaques in Alzheimer's Disease Mouse Model
36.5K Views
11:29
Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
10.7K Views
10:12
Protein Misfolding Cyclic Amplification of Prions
19.4K Views
12:00
Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species
11.0K Views
11:41
Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
7.7K Views
09:50
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
13.5K Views
06:01
Prion Safety Laboratory Swipe Test
448 Views
01:23
Detecting Abnormal Prion Proteins in Brain Tissue Using Immunohistochemistry
93 Views
01:22
Detection of Misfolded Prion Protein Aggregates in Mouse Brain Tissue Using Western Blotting
19 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved