National Academy of Scientists Education program

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

Eliza Guti^*1,2, Ákos Máté Bede^*1,3, Csongor Váróczy^*1,3, Csaba Hegedűs¹, Máté Á. Demény^1,4, László Virág^1,4

¹Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, ²Doctoral School of Molecular Medicine, University of Debrecen, ³National Academy of Scientist Education, University of Debrecen, ⁴ELKH-DE Cell Biology and Signaling Research Group

This protocol presents an automated, image-based high-throughput technique to identify compounds modulating natural killer cell-mediated breast cancer cell killing in the presence of a therapeutic anti-HER-2 antibody.