High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

1.8K Views

13:59 min

August 18th, 2023

DOI :

10.3791/64485-v

August 18th, 2023


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ADCC

Chapters in this video

0:05

Introduction

1:02

Protocol

1:25

Coating of the HCS plate

2:04

Seeding of JIMT-1 EGFP cells

4:33

Pre-treatment of JIMT-1 EGFP cells with compound library

6:06

Starting the ADCC assay by adding the effector cells

9:16

Imaging

10:45

Image analysis

12:24

Representative results

13:15

Discussion

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