University of Perugia

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Ryan S. Edginton¹, Sara Mattana², Silvia Caponi^2,3, Daniele Fioretto², Ellen Green¹, C. Peter Winlove¹, Francesca Palombo¹

¹School of Physics and Astronomy, University of Exeter, ²Department of Physics and Geology, University of Perugia, ³Istituto Officina dei Materiali del CNR, Unità di Perugia

We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

Genetics

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Lorenzo Brunetti^*1,2,3, Michael C. Gundry^*1,2,4, Ayumi Kitano⁴, Daisuke Nakada^1,2,4, Margaret A. Goodell^1,2,4,5

¹Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, ²Center for Cell and Gene Therapy, Baylor College of Medicine, ³Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, ⁴Department of Molecular & Human Genetics, Baylor College of Medicine, ⁵Texas Children's Hospital & Houston Methodist Hospital

A protocol for fast CRISPR/Cas9-mediated gene disruption in mouse and human primary hematopoietic cells is described in this article. Cas9-sgRNA ribonucleoproteins are introduced via electroporation with sgRNAs generated through in vitro transcription and commercial Cas9. High editing efficiencies are achieved with limited time and financial cost.

The Endoscope-Assisted Minimally Invasive Retro-Sigmoid Approach (EAMIRSA): A Combination of Techniques for Improving Middle Skull Base Surgery

Arianna Di Stadio¹, Giampietro Ricci¹, Laura Dipietro³, Puya Dehgani Mobaraki¹, Franco Trabalzini^1,2, Antonio della Volpe^1,4, Luca D'Ascanio^1,5, Jean Jacques Magnan¹

¹Permanent Temporal Bone Dissection Laboratory, University of Perugia, ²Otolaryngology Department, Meyer Children Hospital, ³Highland Instruments, ⁴Otolaryngology Department, Cochlear Implant Unit, Santobono Children Hospital, ⁵Otolaryngology Department, Ospedale Civile of Mantua

The endoscope-assisted minimally invasive retro-sigmoid approach (EAMIRSA) is a surgical technique that can be used for the treatment of the cerebellopontine angle (CPA) and internal auditory canal (IAC) disease. Here, we describe the material necessary to perform EAMIRSA and illustrate the technique using a step-by-step cadaver dissection.