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University of Perugia

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
Ryan S. Edginton 1, Sara Mattana 2, Silvia Caponi 2,3, Daniele Fioretto 2, Ellen Green 1, C. Peter Winlove 1, Francesca Palombo 1
1School of Physics and Astronomy, University of Exeter, 2Department of Physics and Geology, University of Perugia, 3Istituto Officina dei Materiali del CNR, Unità di Perugia

We present a protocol for the application of Brillouin light scattering spectroscopy to elastin and trypsin-purified type I collagen fibers of the extracellular matrix to extract their full elastic properties.

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Genetics

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
Lorenzo Brunetti *1,2,3, Michael C. Gundry *1,2,4, Ayumi Kitano 4, Daisuke Nakada 1,2,4, Margaret A. Goodell 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital

A protocol for fast CRISPR/Cas9-mediated gene disruption in mouse and human primary hematopoietic cells is described in this article. Cas9-sgRNA ribonucleoproteins are introduced via electroporation with sgRNAs generated through in vitro transcription and commercial Cas9. High editing efficiencies are achieved with limited time and financial cost.

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The Endoscope-Assisted Minimally Invasive Retro-Sigmoid Approach (EAMIRSA): A Combination of Techniques for Improving Middle Skull Base Surgery
Arianna Di Stadio 1, Giampietro Ricci 1, Laura Dipietro 3, Puya Dehgani Mobaraki 1, Franco Trabalzini 1,2, Antonio della Volpe 1,4, Luca D'Ascanio 1,5, Jean Jacques Magnan 1
1Permanent Temporal Bone Dissection Laboratory, University of Perugia, 2Otolaryngology Department, Meyer Children Hospital, 3Highland Instruments, 4Otolaryngology Department, Cochlear Implant Unit, Santobono Children Hospital, 5Otolaryngology Department, Ospedale Civile of Mantua

The endoscope-assisted minimally invasive retro-sigmoid approach (EAMIRSA) is a surgical technique that can be used for the treatment of the cerebellopontine angle (CPA) and internal auditory canal (IAC) disease. Here, we describe the material necessary to perform EAMIRSA and illustrate the technique using a step-by-step cadaver dissection.

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