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All India Institute of Medical Sciences, Bhopal

1 ARTICLES PUBLISHED IN JoVE

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Medicine

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry
Kirti Vishwakarma 1, Ayush Gupta 1, Shashank Purwar 1, Navinchandra Motiram Kaore 1, Sunil Tank 1, Shrutika Pundir 2
1Department of Microbiology, All India Institute of Medical Sciences, Bhopal, 2Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar

EUCAST has developed a direct antimicrobial susceptibility testing (AST) protocol for automated blood cultures. However, its dependence on mass spectrometry-based microbial identification can be obviated by using a direct inoculum preparation protocol in an automated microbial identification system. This approach can provide AST reports within 24 h of sample collection.

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