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All India Institute of Medical Sciences, Bhopal

1 ARTICLES PUBLISHED IN JoVE

Medicine

Direct Microbial Identification using An Automated Microbial Identification System to Facilitate the EUCAST RAST Method Without Mass Spectrometry

Kirti Vishwakarma¹, Ayush Gupta¹, Shashank Purwar¹, Navinchandra Motiram Kaore¹, Sunil Tank¹, Shrutika Pundir²

¹Department of Microbiology, All India Institute of Medical Sciences, Bhopal, ²Department of Microbiology, All India Institute of Medical Sciences, Bhubaneswar

EUCAST has developed a direct antimicrobial susceptibility testing (AST) protocol for automated blood cultures. However, its dependence on mass spectrometry-based microbial identification can be obviated by using a direct inoculum preparation protocol in an automated microbial identification system. This approach can provide AST reports within 24 h of sample collection.