In Vivo Leaf Inoculation: An Alternative Method to Assess the Disease Resistance of Hybrid Clones in Poplar Breeding of Stem Canker DiseaseYutian Suo *1,2, Yinan Zhang *1,2, Zheng Li 1,2, Mingzhuo Zhao 3, Wanna Shen 1,2, Yuchen Fu 1,2, Donglin Wang 1,2, Long Pan 1,2, Xiaohua Su 4, Jiaping Zhao 1,2
1State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, 2Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, 3Beijing Haidian Kaiwen Academy High School, 4State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry
We provide a step-by-step protocol for assessing poplar resistance to stem canker pathogens using an in vivo leaf inoculation method. This method is especially suitable for large-scale assessment of Cytospora chrysosperma and Botryosphaeria dothidea canker disease resistance in poplar breeding progeny in China.
