In Vivo Leaf Inoculation: An Alternative Method to Assess the Disease Resistance of Hybrid Clones in Poplar Breeding of Stem Canker Disease

529 Views

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09:31 min

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September 20th, 2024

DOI :

10.3791/67290-v

September 20th, 2024

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Yutian Suo*¹^,², Yinan Zhang*¹^,², Zheng Li¹^,², Mingzhuo Zhao³, Wanna Shen¹^,², Yuchen Fu¹^,², Donglin Wang¹^,², Long Pan¹^,², Xiaohua Su⁴, Jiaping Zhao¹^,²

¹State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, ²Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry, ³Beijing Haidian Kaiwen Academy High School, ⁴State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry

Summary

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In Vivo Leaf Inoculation

Disease Resistance Assessment

Hybrid Clones

Poplar Breeding

Stem Canker Disease

Botryosphaeria Dothidea

Cytospora Chrysosperma

Disease Resistance Genes

Molecular Breeding

Hybridization

Genetic Analysis

Pathogen Inoculation Method

High Throughput

Poplar Populations

Chapters in this video

0:00

Introduction

2:20

Preparing Reagents and Poplar Material for In Vivo Leaf Inoculation

4:32

In Vivo Inoculation of Stem Canker Pathogens in Poplar Leaves to Test the Canker Disease Resistance

6:14

Leaf Pathotype Acquisition and Assessment of Poplar Resistance to Stem Canker Diseases in the Laboratory

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