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Universidad Autónoma de Madrid

5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology
Anna Castells-Nobau *1, Bonnie Nijhof *1, Ilse Eidhof 1, Louis Wolf 2, Jolanda M. Scheffer-de Gooyert 1, Ignacio Monedero 3,4, Laura Torroja 3, Jeroen A.W.M. van der Laak 2,5, Annette Schenck *1
1Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, 2Microscopical Imaging Centre (MIC), Radboud University Medical Center, 3Department of Biology, Universidad Autónoma de Madrid, 4Department of Clinical and Experimental Medicine, Linköping University, 5Department of Pathology, Radboud University Medical Center

Two image analysis algorithms, "Drosophila NMJ Morphometrics" and "Drosophila NMJ Bouton Morphometrics" were created, to automatically quantify nine morphological features of the Drosophila neuromuscular junction (NMJ).

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Chemistry

Microfluidic-based Synthesis of Covalent Organic Frameworks (COFs): A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface
Afshin Abrishamkar 1, David Rodríguez-San-Miguel 2, Jorge Andrés Rodríguez Navarro 3, Romen Rodriguez-Trujillo 4, David B. Amabilino 5, Ruben Mas-Ballesté 2, Félix Zamora 2,6,7, Andrew J. deMello 1, Josep Puigmarti-Luis 1
1Institute of Chemical and Bioengineering, Department of Chemistry and Applied Bioscience, ETH Zurich, 2Departamento de Química Inorgánica, Universidad Autónoma de Madrid, 3Departamento de Química Inorgánica, Universidad de Granada, 4Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), 5School of Chemistry, University of Nottingham, 6Condensed Matter Physics Center (IFMAC), Universidad Autónoma de Madrid, 7Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia)

We present a novel microfluidic-based method for synthesis of covalent organic frameworks (COFs). We demonstrate how this approach can be used to produce continuous COF fibers, and also 2D or 3D COF structures on surfaces.

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Environment

BtM, a Low-cost Open-source Datalogger to Estimate the Water Content of Nonvascular Cryptogams
María Leo *1, Angel Lareo *2, Carlos Garcia-Saura 2, Joaquín Hortal 3,4, Nagore G. Medina 5
1Real Jardín Botánico (CSIC-RJB), 2Grupo de Neurocomputación Biológica, Dpto. de Ingeniería Informática, Escuela Politécnica Superior, Universidad Autónoma de Madrid, 3Department of Biogeography and Global Change, Museo Nacional de Ciencias Naturales (MNCN-CSIC), 4Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências da Universidade de Lisboa, 5Department of Botany, Faculty of Science, University of South Bohemia

We present a simple and cost-effective method to build an open-source datalogger that measures the conductance of nonvascular cryptogams together with the environmental temperature and humidity. We describe the hardware design of the datalogger and provide step-by-step assembly instructions, the list of required open-source logging software, the code to run the datalogger, and a calibration protocol.

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Immunology and Infection

Imaging the Human Immunological Synapse
Ana Bello-Gamboa *1,2, Juan Manuel Izquierdo 1,2, Marta Velasco 1,2, Solange Moreno 1,2, Alejandro Garrido 1,2, Laura Meyers 1,2, Juan Carlos Palomino 3, Víctor Calvo 1,2, Manuel Izquierdo *1,2
1Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-Universidad Autónoma de Madrid, 2Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina, Universidad Autónoma de Madrid, 3Unidad de Audiovisuales, Facultad de Medicina, Universidad Autónoma de Madrid

This protocol images both the immunological synapse formation and the subsequent polarized secretory traffic towards the immunological synapse. Cellular conjugates were formed between a superantigen-pulsed Raji cell (acting as an antigen-presenting cell) and a Jurkat clone (acting as an effector helper T lymphocyte).

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Neuroscience

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration
María Portela-Lomba 1,3, Diana Simón 1, Cristina Russo 2, Javier Sierra 1, María Teresa Moreno-Flores 3
1Facultad de CC Experimentales, Universidad Francisco de Vitoria, 2Dept. Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, 3Dept. Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid

We present an in vitro model to assess olfactory ensheathing glia (OEG) neuroregenerative capacity, after neural injury. It is based on a coculture of axotomized adult retinal ganglion neurons (RGN) on OEG monolayers and subsequent study of axonal regeneration, by analyzing RGN axonal and somatodendritic markers.

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