Imaging the Human Immunological Synapse

1:26

Adhesion of Raji Cells to the Chamber Slides and 7-amino-4-chloromethylcoumarin (CMAC, Cell Tracker Blue) Labeling

3:31

Pulse of CMAC labeled Raji Cells with Staphylococcal Enterotoxin E and Preparation of Jurkat Cells

4:51

Co-seeding of Raji and Jurkat Cells and Time Lapse Imaging of Emerging Synaptic Conjugates

5:51

End Point Formation of Synaptic Conjugates and Fixation

6:38

Results: Imaging the Human Immunological Synapse

8:28

Conclusion