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Universidad de Buenos Aires

4 ARTICLES PUBLISHED IN JoVE

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Behavior

Probing the Limits of Egg Recognition Using Egg Rejection Experiments Along Phenotypic Gradients
Lindsay Canniff 1, Miri Dainson 2, Analía V. López 3, Mark E. Hauber 2, Tomáš Grim 4, Peter Samaš 5, Daniel Hanley 1
1Department of Biology, Long Island University-Post, 2Department of Animal Biology, School of Integrative Biology, University of Illinois, 3Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 4Department of Zoology and Laboratory of Ornithology, Palacký University, 5Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic

This protocol provides guidelines for running egg rejection experiments: outlining techniques for painting experimental egg models to emulate the colors of natural bird eggs, conducting fieldwork, and analyzing the collected data. This protocol provides a uniform method for conducting comparable egg rejection experiments.

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Bioengineering

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Marcos Ezequiel Gramajo *1, Ryan J. Lake *2, Yi Lu 3, Ana Sol Peinetti 1
1INQUIMAE (CONICET), Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2Department of Chemistry, University of Illinois at Urbana-Champaign, 3Department of Chemistry, University of Texas at Austin

We provide a protocol that can be generally applied to select aptamers that bind to infectious viruses only and not to viruses that have been rendered non-infectious by a disinfection method or to any other similar viruses. This opens the possibility of determining infectivity status in portable and rapid tests.

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Medicine

An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
Florencia S. Fontana Estevez *1, Verónica Miksztowicz *1, Ignacio M. Seropián 1,2, Pablo Cassaglia 3, Romina Bustos 1, Vanessa Touceda 1, Tomas Cianciulli 4, Verónica Cassanova 5, Celina Morales 3, Germán E. González 1
1Laboratorio de Patología Cardiovascular Experimental e Hipertensión Arterial, Instituto de Investigaciones Biomédicas, Universidad Católica Argentina-CONICET, 2Servicio de Hemodinamia y Cardiología Intervencionista, Hospital Italiano de Buenos Aires, 3Instituto de Fisiopatologia Cardiovascular, Facultad de Medicina, Universidad de Buenos Aires, 4División Cardiología, Hospital General de Agudos "Dr. Cosme Argerich,", 5Facultad de Veterinaria, Universidad de Buenos Aires

Here, we describe an experimental model of myocardial infarction, an echocardiography procedure to study cardiac remodeling and function, and procedures for quantifying fibrosis and hypertrophy in picrosirius red-stained and rhodamine-stained sections, as well as the infarct size and expansion index in slices stained with Masson's trichrome.

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Developmental Biology

Early Unguided Human Brain Organoid Neurovascular Niche Modeling into the Permissive Chick Embryo Chorioallantoic Membrane
Luciano Fiore 1,2, Jan Arderiu 3, Andrea Martí-Sarrias 3,4, Isabel Turpín 3,4, Ruth I. Pareja 3,5, Arcadi Navarro 5,6,7,8, Mariana Holubiec 2,9, Julieta Bianchelli 9, Tomas Falzone 2,9, Gonzalo Spelzini 1,2, Gabriel Scicolone 1,2, Sandra Acosta 3,4
1Instituto de Biología Celular y Neurociencias “Prof. E. De Robertis” (IBCN), CONICET - Universidad de Buenos Aires, 2Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética, Universidad de Buenos Aires, 3Institute of Neurosciences, Pathology and Experimental Therapeutics Dept, University of Barcelona, 4Functional Neurogenomics Group, Neurodevelopmental Disorders, IDIBELL, L’Hospitalet de Llobregat, 5IBE, Institute of Evolutionary Biology (UPF-CSIC), Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 6Institució Catalana de Recerca i Estudis Avançats (ICREA) and Universitat Pompeu Fabra, 7Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 8BarcelonaBeta Brain Research Center, Pasqual Maragall Foundation, 9Instituto de Investigación en Biomedicina (IBioBA) – CONICET – Instituto Partner de la Sociedad Max Planck

Here, we present a protocol to engraft human brain organoids at multiple maturation stages into the chick chorioallantoic membrane (CAM). Brain organoids were grown following unguided standardized protocols.

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