Louisiana State University Agricultural Center

3 ARTICLES PUBLISHED IN JoVE

Biology

Bioassays for Monitoring Insecticide Resistance

Audra L.E. Miller¹, Kelly Tindall¹, B. Rogers Leonard²

¹Division of Plant Sciences, University of Missouri, Delta Research Center, ²Department of Entomology, Louisiana State University Agricultural Center

This manuscript demonstrates and discusses techniques used to survey pesticide susceptibility and detect resistance to contact and systemic pesticides in arthropod pests.

Biology

Testing Protozoacidal Activity of Ligand-lytic Peptides Against Termite Gut Protozoa in vitro (Protozoa Culture) and in vivo (Microinjection into Termite Hindgut)

Claudia Husseneder^*1, Amit Sethi^*1, Lane Foil¹, Jennifer Delatte¹

¹Department of Entomology, Louisiana State University Agricultural Center

We present procedures for demonstrating that ligands bind to the surface membrane of the cellulose-digesting protozoa in the gut of Formosan subterranean termites using fluorescent microscopy and that ligands coupled with lytic peptides kill these protozoa in vitro (anaerobic protozoa culture) and in vivo (injection into the termite hindgut).

Environment

Assessment of Labile Organic Carbon in Soil Using Sequential Fumigation Incubation Procedures

Michael A. Blazier¹, Hal O. Liechty²

¹Hill Farm Research Station, Louisiana State University Agricultural Center, ²School of Forestry and Natural Resources, University of Arkansas at Monticello

Labile organic carbon (LOC) and the potential carbon turnover rate are sensitive indicators of changes in soil nutrient cycling processes. Details are provided for a method based on fumigating and incubating soil in a series of cycles and using the CO₂ accumulated during the incubation periods to estimate these parameters.