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Maine Medical Center Research Institute

6 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
Aaron C. Brown 1, Ulrika Blank 2, Derek C. Adams 1, Michele J. Karolak 1, Jennifer L. Fetting 1, Beth L. Hill 1, Leif Oxburgh 1
1Department of Molecular Medicine, Maine Medical Center Research Institute, 2Molecular Medicine and Gene Therapy, Lund University Hospital

In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.

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Developmental Biology

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
David E. Maridas 1, Elizabeth Rendina-Ruedy 1, Phuong T. Le 1, Clifford J. Rosen 1
1Maine Medical Center Research Institute

In this article, we present methods to isolate and differentiate bone marrow stromal cells and hematopoietic stem cells from mouse long bones. Two different protocols are presented yielding different cell populations suitable for expansion and differentiation into osteoblasts, adipocytes, and osteoclasts.

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Biology

Differentiation Capacity of Human Aortic Perivascular Adipose Progenitor Cells
S. Spencer Scott 1, Xuehui Yang 1, Michael Robich 1, Lucy Liaw 1, Joshua M. Boucher 1
1Maine Medical Center Research Institute

The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages.

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Biology

Transverse Fracture of the Mouse Femur with Stabilizing Pin
Emily R. Moore 1, Marina Feigenson 2, David E. Maridas 1
1Department of Developmental Biology, Harvard School of Dental Medicine, 2Keros Therapeutics

This protocol describes a method to perform fractures on adult mice and monitor the healing process.

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Biology

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
Shobana Jayapalan 1,2, Ananya Nandy 1,2, Elizabeth Rendina-Ruedy 1,2,3
1Vanderbilt Center for Bone Biology, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 3Department of Molecular Physiology and Biophysics, Vanderbilt University

Real-time cell metabolic flux assay measures the oxygen consumption rate and extracellular acidification rate, which corresponds to mitochondrial and glycolytic adenosine triphosphate production, using pH and oxygen sensors. The manuscript explains a method to understand the energy status of osteoblasts and the characterization and interpretation of the cellular bioenergetic status.

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Methods For Studying Osteoenergetics And Metabolism
Elizabeth Rendina-Ruedy 1, Courtney M. Karner 2
1Department of Medicine, Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Internal Medicine, Charles and Jane Pak Center for Mineral Metabolism and Clinical Research, University of Texas Southwestern Medical Center

Methods For Studying Osteoenergetics And Metabolism

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