Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
3.2K Views
•
09:43 min
•
March 1st, 2022
DOI :
March 1st, 2022
•Chapters in this video
0:04
Introduction
0:23
Preparation of Compounds for the Sensor Cartridges
1:42
Prepare Cell Culture Microplate for Assay
2:34
Setting up the Assay and Imaging
3:44
Obtain Brightfield Images
4:59
Running the Assay
6:13
Obtain Fluorescence Images and Normalize
7:18
Results: Reactivity of Osteoblasts Control Versus Treatment Groups Against Inhibitors
9:15
Conclusion
Related Videos
07:45
Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis
35.4K Views
07:20
Bioenergetic Profile Experiment using C2C12 Myoblast Cells
50.4K Views
07:36
Autofluorescence Imaging to Evaluate Cellular Metabolism
4.1K Views
A Comprehensive High-Efficiency Protocol for Isolation, Culture, Polarization, and Glycolytic Characterization of Bone Marrow-Derived Macrophages
3.8K Views
09:39
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
1.8K Views
06:26
Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes
184 Views
10:17
Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
2.6K Views
Evaluation of Fatty Acid Oxidation in Cultured Bone Cells
741 Views
09:16
Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
2.3K Views
04:39
Extracellular Flux Assay: A Method for Measuring Metabolic Profile of Cells
1.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved