Sign In

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

3.1K Views

09:43 min

March 1st, 2022

DOI :

10.3791/63142-v

March 1st, 2022


Explore More Videos

Real Time Cell Metabolic Flux Analyzer

Chapters in this video

0:04

Introduction

0:23

Preparation of Compounds for the Sensor Cartridges

1:42

Prepare Cell Culture Microplate for Assay

2:34

Setting up the Assay and Imaging

3:44

Obtain Brightfield Images

4:59

Running the Assay

6:13

Obtain Fluorescence Images and Normalize

7:18

Results: Reactivity of Osteoblasts Control Versus Treatment Groups Against Inhibitors

9:15

Conclusion

Related Videos

article

14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

11.0K Views

article

09:21

Quantitative Real-Time PCR using the Thermo Scientific Solaris qPCR Assay

49.6K Views

article

07:37

Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

10.9K Views

article

09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

22.5K Views

article

08:53

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

16.1K Views

article

09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

9.0K Views

article

06:31

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

8.9K Views

article

09:16

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

15.3K Views

article

08:21

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

12.8K Views

article

06:29

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

3.0K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved