uib

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Labeling of Extracellular Vesicles for Monitoring Migration and Uptake in Cartilage Explants

Maria Antònia Forteza-Genestra^1,2, Miquel Antich-Rosselló^1,2, Francisco Gabriel Ortega^1,2, Guillem Ramis-Munar³, Javier Calvo^1,2,4, Antoni Gayà^1,2,4, Marta Monjo^1,2, Joana Maria Ramis^1,2

¹Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, ²Health Research Institute of the Balearic Islands (IdISBa), ³Cellomics Unit, Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, ⁴Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB)

Here, we present a protocol to label platelet lysate-derived extracellular vesicles to monitor their migration and uptake in cartilage explants used as a model for osteoarthritis.

Bioengineering

Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants

Miquel Antich-Rosselló^1,2, Maria Antònia Forteza-Genestra^1,2, Javier Calvo^1,2,3, Antoni Gayà^1,2,3, Marta Monjo^1,2,4, Joana Maria Ramis^1,2,4

¹Cell Therapy and Tissue Engineering Group, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, ²Health Research Institute of the Balearic Islands (IdISBa), ³Fundació Banc de Sang i Teixits de les Illes Balears (FBSTIB), ⁴Department of Fundamental Biology and Health Sciences, University of the Balearic Islands

Here, we present a method for the isolation of Extracellular Vesicles (EVs) derived from the platelet lysates (PL) and their use for coating titanium (Ti) implant surfaces. We describe the drop casting coating method, the EVs release profile from the surfaces, and in vitro biocompatibility of EVs coated Ti surfaces.

Immunology and Infection

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Marta Munar-Bestard^1,2, Oscar Villa¹, Maria del Mar Ferrà-Cañellas^1,2,3, Joana Maria Ramis^1,2,4,5, Marta Monjo^1,2,4,5

¹Group of Cell Therapy and Tissue Engineering, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands (UIB), ²Balearic Islands Health Research Institute (IdISBa), ³Preclinical Research Department, Labo'Life España, ⁴Departament de Biologia Fonamental i Ciències de la Salut, UIB, ⁵ADEMA School of Dentistry, UIB

In this study, a rat model of induction of periodontitis is presented via a combination of retentive ligature and repetitive injections of lipopolysaccharide derived from Porphyromonas gingivalis, over 14 days around the first maxillary molars. The ligation and LPS injection techniques were effective in inducing peridontitis, resulting in alveolar bone loss and inflammation.