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Odense University Hospital

10 ARTICLES PUBLISHED IN JoVE

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Medicine

Evaluating the Procedure for Performing Awake Cystometry in a Mouse Model
Travis K. Mann-Gow *1, Troy R. Larson *1, Chrissie T. Wøien 2, Thomas M. Andersen 2, Karl-Erik Andersson 3,4, Peter Zvara 1,2
1Department of Surgery, University of Vermont, 2Department of Urology and Biomedical Laboratory, University of Southern Denmark, 3Wake Forest Institute for Regenerative Medicine, Wake Forest University, 4Institute for Clinical Medicine, Aarhus University

This study describes the surgical procedures and experimental techniques for performing awake cystometry in a freely moving mouse. In addition, it provides experimental evidence to support its optimization and standardization.

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Medicine

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat
Morten Hox 1, Travis Mann-Gow 2, Lars Lund 3,4, Peter Zvara 1,4
1Biomedical Laboratory and the Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, 2Department of Surgery, University of Vermont, 3Research Unit of Urology, Department of Clinical Research, University of Southern Denmark, 4Department of Urology, Odense University Hospital

This study describes a simplified surgical procedure and technique for performing cavernous nerve stimulation with the isolation of the nerve-electrode complex using silicone glue and intracavernous pressure measurement.

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JoVE Journal

Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy
Maria Bloksgaard 1, Bjarne Thorsted 2, Jonathan R. Brewer 2, Jo G. R. De Mey 1,3
1Department of Cardiovascular and Renal Research, Institute of Molecular Medicine, University of Southern Denmark, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3Department of Cardiac, Thoracic and Vascular Surgery, Odense University Hospital

We describe simultaneous mechanical testing and 3D-imaging of the arterial wall of isolated, live human resistance arteries, and Fiji and Ilastik image analyses for the quantification of elastin and collagen spatial organization and volume densities. We discuss the use of these data in mathematical models of arterial wall mechanics.

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Medicine

Isolation of Adipose Derived Regenerative Cells for the Treatment of Erectile Dysfunction Following Radical Prostatectomy
Sabrina T. Hansen 1,2, Charlotte H. Jensen 2,3,4, Jens A. Sørensen 2,4,5, Søren P. Sheikh 2,3,4, Lars Lund 1,2,4
1Department of Urology, Odense University Hospital, 2Clinical Institute, University of Southern Denmark, 3Laboratory of Molecular and Cellular Cardiology, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, 4Center for Vascular Regeneration, Odense University Hospital, 5Research Unit for Plastic Surgery, Odense University Hospital

Precise disclosure of methods and protocols is crucial for large scale uptake of stem cell therapies. Here, we present a protocol to isolate adipose-derived regenerative cells, used for a single intracavernous injection as treatment of erectile dysfunction (ED) following radical prostatectomy (RP).

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Immunology and Infection

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
Jeff Granhøj 1, Henrik Dimke 1,2, Per Svenningsen 1
1Department of Molecular Medicine, University of Southern Denmark, 2Department of Nephrology, Odense University Hospital

Here we present a method to select for novel variants of the E. coli biotin-protein ligase BirA that biotinylates a specific target peptide. The protocol describes the construction of a plasmid for the bacterial display of the target peptide, generation of a BirA library, selection and characterization of BirA variants.

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Medicine

A Revised Method for Inducing Secondary Lymphedema in the Hindlimb of Mice
Alexander Wiinholt 1,2, Mads G. Jørgensen 2, Amar Bučan 1,2, Farima Dalaei 1,2, Jens A. Sørensen 2
1University of Southern Denmark, 2Department of Plastic Surgery, Odense University Hospital

This animal model enables researchers to induce statistically significant secondary lymphedema in the hindlimb of mice, lasting at least 8 weeks. The model can be used to study the pathophysiology of lymphedema and to investigate novel treatment options.

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Medicine

Radial Endobronchial Ultrasound and Electromagnetic Navigation Bronchoscopy with Fluoroscopy for the Diagnosis of Peripheral Lung Lesions
Amanda Dandanell Juul 1,2, Alexis Pulga 3, Arman Arshad 1
1Department of Respiratory Medicine, Odense University Hospital, 2Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, 3Department of Respiratory Medicine, Bispebjerg Hospital

Diagnosing small lung tumors is quite difficult using a bronchoscope alone. Electromagnetic navigation bronchoscopy is used to locate the lesion, similar to the Global Positioning System. Radial endobronchial ultrasound and fluoroscopy confirm the correct location and monitor the sampling.

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Medicine

Using the Endoscope for Endobronchial Ultrasound in the Esophagus
Mohammad A. Issa 1, Paul F. Clementsen 2, Christian B. Laursen 3,4, Peter Vilmann 5,6, Ida S. Christiansen 7, Laurence Crombag 8, Uffe Bodtger 1,9
1Pulmonary Research Unit (PLUZ), Department of Respiratory Medicine, Zealand University Hospital, 2Copenhagen Academy for Medical Education and Simulation (CAMES), Copenhagen University Hospital, Rigshospitalet, 3Department of Respiratory Medicine, Odense University Hospital, 4Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, 5Department of Surgery, Copenhagen University Hospital, 6Department of Clinical Medicine, University of Copenhagen, 7Department of Pathology, Copenhagen University Hospital, Rigshospitalet, 8Department of Respiratory Medicine, University Medical Center, University of Amsterdam, 9Institute for Regional Health Research, University of Southern Denmark

Transesophageal ultrasound (EUS-B) is a safe and feasible procedure using the echoendobronchoscope (EBUS) in esophagus and stomach. After identifying six anatomical landmarks, additional structures can be identified and biopsied, sparing subsequent diagnostic sessions. Thus, EUS-B is an ideal continuation of bronchoscopy and EBUS in diagnosing lung cancer and other diseases.

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Medicine

Transbronchial Lung Cryobiopsy for Diagnosing Interstitial Lung Diseases and Peripheral Pulmonary Lesions - A Stepwise Approach
Jesper R. Davidsen 1,2, Christian B. Laursen 1,2, Søren H. Skaarup 3, Sissel B. Kronborg-White 3, Amanda D. Juul 1,2
1South Danish Center for Interstitial Lung Diseases (SCILS), Department of Respiratory Medicine, Odense University Hospital, 2Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, 3Department of Respiratory Diseases and Allergy, Aarhus University Hospital

Transbronchial lung cryobiopsy (TBLC) for diagnosing interstitial lung disease and peripheral pulmonary lesions is a high-yield diagnostic and safe procedure. We describe a stepwise approach to conduct TBLC for the different indications mentioned with a flexible bronchoscope, which might be helpful for novice bronchoscopists performing TBLC.

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Medicine

A Stepwise Approach for Performing Ultrasound Guided Transthoracic Lung Biopsy
Christian B. Laursen 1,2, Rahul Bhatnagar 2,3,4, Amanda Dandanell Juul 1,2
1Department of Respiratory Medicine, Odense University Hospital, 2Odense Respiratory Research Unit (ODIN), Department of Clinical Research, University of Southern Denmark, 3Respiratory Medicine Department, North Bristol NHS Trust, Southmead Hospital, 4Academic Respiratory Unit, University of Bristol

Transthoracic ultrasound-guided lung biopsy represents a safe, cost-effective, and efficient approach for patients presenting with subpleural lung lesions suspected of malignancy. Employing a systematic, step-by-step process is crucial to achieve optimal patient selection, minimize complication risks, and maximize diagnostic accuracy.

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