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University of Barcelona

11 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
Sílvia Bofill-Mas 1, Ayalkibet Hundesa 1, Byron Calgua 1, Marta Rusiñol 1, Carlos Maluquer de Motes 1, Rosina Girones 1
1Laboratory of Water and Food Viral Pollution, Department of Microbiology, Faculty of Biology, University of Barcelona

The study describes a cost-effective method for the identification of the source of fecal/urine contamination or contamination by nitrates in water using qPCR for the specific quantification of human/porcine/bovine DNA viruses, adenoviruses and polyomaviruses, proposed as MST tools.

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Medicine

An Anatomical Study of Nerves at Risk During Minimally Invasive Hallux Valgus Surgery
Miki Dalmau-Pastor 1,2,3, Jordi Vega 1,4, Francesc Malagelada 1,5, Fernando Peña 6, Maria Cristina Manzanares-Céspedes 1
1Laboratory of Arthroscopic and Surgical Anatomy. Department of Pathology and Experimental Therapeutics (Human Anatomy and Embryology Unit), University of Barcelona, 2Health Sciences Faculty of Manresa, University of Vic-Central University of Catalunya, 3Groupe de Recherche et d'Etude en Chirurgie Mini-Invasive du Pied, GRECMIP, 4Foot and Ankle Unit, Hospital Quirón Barcelona, 5Foot and Ankle Unit, Orthopedic and Trauma Surgery, Royal London Hospital, Barts Health NHS Trust, 6Department of Orthopedic Surgery, Foot and Ankle Unit, University of Minnesota

Minimally invasive surgical (MIS) procedures rely on anatomical references to localize structures not directly visible to the surgeon. This manuscript describes a combined method of plane-by-plane dissection and sectional anatomy of fresh-frozen specimens to locate the structures at risk during MIS procedures.

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Environment

Cereal Crop Ear Counting in Field Conditions Using Zenithal RGB Images
José A. Fernandez-Gallego 1,2,3, María Luisa Buchaillot 1,2, Adrian Gracia-Romero 1,2, Thomas Vatter 1,2, Omar Vergara Diaz 1,2, Nieves Aparicio Gutiérrez 4, María Teresa Nieto-Taladriz 5, Samir Kerfal 6, Maria Dolors Serret 1,2, José Luis Araus 1,2, Shawn C. Kefauver 1,2
1Plant Physiology Section, Faculty of Biology, University of Barcelona, 2Agrotecnio, 3Programa de Ingeniería Electrónica, Facultad de Ingeniería, Universidad de Ibagué, 4Instituto Tecnológico Agrario de Castilla y León (ITACyL), 5Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 6Syngenta Spain

We present a protocol for counting durum wheat and barley ears, using natural color (RGB) digital photographs taken in natural sunlight under field conditions. With minimal adjustments for camera parameters and some environmental condition limitations, the technique provides precise and consistent results across a range of growth stages.

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Immunology and Infection

VirWaTest, A Point-of-Use Method for the Detection of Viruses in Water Samples
David Aguado 1, Eva Fores 1, Laura Guerrero-Latorre 1,2, Marta Rusiñol 1, Sandra Martínez-Puchol 1, Francesc Codony 3,4, Rosina Girones 1, Sílvia Bofill-Mas 1
1Laboratory of Viruses Contaminants of Water and Food (VIRCONT), Department of Genetics, Microbiology and Statistics, Section of Microbiology, Virology and Biotechnology, University of Barcelona, 2Grupo de Investigación Biodiversidad, Medio Ambiente y Salud (BIOMAS), Facultad de Ingenierías y Ciencias Agropecuarias (FICA), Ingeniería en Biotecnología, Universidad de las Américas, 3Municipal Laboratory - Waters of Mataró, 4GenIUL

Here we present VirWaTest, which is a simple, affordable and portable method for the concentration and detection of viruses from water samples at the point of use.

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Neuroscience

Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
Vanessa Gil 1,2,3,4, José Antonio Del Río 1,2,3,4
1Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Parc Científic de Barcelona, 2Department of Cell Biology, Physiology, and Immunology, Universitat de Barcelona, 3Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), 4Institute of Neuroscience, University of Barcelona

Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.

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Neuroscience

Meta-analysis of Voxel-Based Neuroimaging Studies using Seed-based d Mapping with Permutation of Subject Images (SDM-PSI)
Anton Albajes-Eizagirre 1,2, Aleix Solanes 1,2, Miquel Angel Fullana 2,3, John P. A. Ioannidis 4, Paolo Fusar-Poli 5,6,7, Carla Torrent 1,2,3,8, Brisa Solé 1,2,3,8, Caterina Mar Bonnín 1,2,3,8, Eduard Vieta 1,2,3,8, David Mataix-Cols 9, Joaquim Radua 1,2,5,9
1Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 2Mental Health Research Networking Center (CIBERSAM), 3Institute of Neurosciences, Hospital Clinic de Barcelona, 4Departments of Medicine, of Health Research and Policy, and of Biomedical Data Science, Stanford University School of Medicine, and Department of Statistics, Stanford University School of Humanities and Sciences, 5Department of Psychosis Studies, Institute of Psychiatry, Psychology and Neuroscience, King's College London, 6OASIS Service, South London and Maudsley NHS Foundation Trust, 7Department of Nervous System and Behavioral Sciences, University of Pavia, 8University of Barcelona, 9Centre for Psychiatric Research and Education, Department of Clinical Neuroscience, Karolinska Institutet

We detail how to conduct a meta-analysis of voxel-based neuroimaging studies using Seed-based d Mapping with Permutation of Subject Images (SDM-PSI).

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Cancer Research

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
Joan Repullés 1,2, Mariona Terradas 1,3,4, Gemma Fuster 5,6,7, Anna Genescà 1, Teresa Anglada 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

This protocol provides experimental in vitro tools to evaluate the transformation of human mammary cells. Detailed steps to follow-up cell proliferation rate, anchorage-independent growth capacity, and distribution of cell lineages in 3D cultures with basement membrane matrix are described.

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Engineering

Glass-Based Devices to Generate Drops and Emulsions
Josefa Guerrero 1, Javier Rojo 2, Alexis de la Cotte 2, Luis Manuel Aguilera-Sáez 3, Enric Vila 3, Alberto Fernandez-Nieves 2,4,5
1Department of Chemistry and Physics, Augusta University, 2Department of Condensed Matter Physics, University of Barcelona, 3Agrobío S.L., 4ICREA - Institució Catalana de Recerca i Estudis Avançats, 5Institute of Complex Systems (UBICS), University of Barcelona

Here, a protocol to manufacture glass-based microfluidic devices used for generating highly monodisperse emulsions with controlled drop size is presented.

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Developmental Biology

Early Unguided Human Brain Organoid Neurovascular Niche Modeling into the Permissive Chick Embryo Chorioallantoic Membrane
Luciano Fiore 1,2, Jan Arderiu 3, Andrea Martí-Sarrias 3,4, Isabel Turpín 3,4, Ruth I. Pareja 3,5, Arcadi Navarro 5,6,7,8, Mariana Holubiec 2,9, Julieta Bianchelli 9, Tomas Falzone 2,9, Gonzalo Spelzini 1,2, Gabriel Scicolone 1,2, Sandra Acosta 3,4
1Instituto de Biología Celular y Neurociencias “Prof. E. De Robertis” (IBCN), CONICET - Universidad de Buenos Aires, 2Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética, Universidad de Buenos Aires, 3Institute of Neurosciences, Pathology and Experimental Therapeutics Dept, University of Barcelona, 4Functional Neurogenomics Group, Neurodevelopmental Disorders, IDIBELL, L’Hospitalet de Llobregat, 5IBE, Institute of Evolutionary Biology (UPF-CSIC), Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 6Institució Catalana de Recerca i Estudis Avançats (ICREA) and Universitat Pompeu Fabra, 7Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 8BarcelonaBeta Brain Research Center, Pasqual Maragall Foundation, 9Instituto de Investigación en Biomedicina (IBioBA) – CONICET – Instituto Partner de la Sociedad Max Planck

Here, we present a protocol to engraft human brain organoids at multiple maturation stages into the chick chorioallantoic membrane (CAM). Brain organoids were grown following unguided standardized protocols.

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Medicine

Surgical Bone Implantation Technique for Rat Tibia Models of Diabetes and Osteoporosis
David Alfaiate 1,2, Carlos Mas-Moruno 3,4, Cidália Manuela 5, Josep Maria Ustrell 6,7, Juan Antonio Camara 8,9, Marina Ferrer 10,11, José Maria Manero 3,4, Maria-Cristina Manzanares-Céspedes 12
1Faculty of Medicine and Health Sciences, University of Barcelona, 2Department of Oral Surgery, Davallmed Clinic. Porto, 3Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya, 4Barcelona Research Centre in Multiscale Science and Engineering, Universitat Politècnica de Catalunya, 5Department of Periodontology, Davallmed Clinic. Porto, 6Department of Odontostomatology, University of Barcelona, 7Oral Health and Masticatory System Research Group at the Bellvitge Biomedical Research Institute (IDIBELL), 8Preclinical imaging platform, Vall d'Hebron Research Institute, Barcelona, 9Preclinical Therapeutics Core, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Animal facility, Vall d'Hebron Research Institute, Barcelona, 11UCSF Gnotobiotics Core Facility, University of California, San Francisco, 12Human Anatomy and Embryology Unit, Experimental Pathology and Therapeutics Department, Faculty of Medicine and Health Sciences, University of Barcelona

The placement of implants in a rat model is an essential experimental procedure for clinical research. This study presents a comprehensive surgical protocol for implanting titanium implants into the tibia of rat models with diabetes and osteoporosis.

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Chemistry

Synthesis of Black Seed Oil-loaded-, Alginate-based, pH-sensitive Beads Using Electrospraying Technique
Abul Kalam Azad 1, Mohamad Dayoob 2, Vinoth Kumarasamy 3, Vetriselvan Subramaniyan 4, Ghadeer M. Albadrani 5, Muath Q. Al-Ghadi 6, Mohamed Kamel 7, Mohamed M. Abdel-Daim 8,9, Mireia Mallandrich Miret 10, Abd Almonem Doolaanea 11,12
1Faculty of Pharmacy, University College of MAIWP International, 2Faculty of Pharmacy, MAHSA University, 3Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 4Department of Medical Sciences, School of Medical and Life Sciences, Sunway University, 5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, 6Department of Zoology, College of Science, King Saud University, 7Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 8Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, 9Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, 10Department of Pharmacy, Pharmaceutical Technology and Physical-Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 11Research and Development, Sabrena Experience, 12Alphastar Lab Systems

A technique employing high electric voltage and a targeted, active ingredient-loaded emulsion to fabricate pH-responsive, uniform microbeads is presented.

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