Institut de recherches cliniques de Montréal

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Sébastien D. Langlois^*1,2, Steves Morin^*1, Patricia T. Yam^1,3,4, Frédéric Charron^1,2,5,6,7

¹Molecular Biology of Neural Development, Institut de Recherches Cliniques de Montréal, ²Division of Experimental Medicine and Program in Neuroengineering, McGill University, ³Program in Neuroengineering, McGill University, ⁴Montreal Neurological Institute, ⁵Department of Anatomy and Cell Biology, McGill University, ⁶Department of Biology, McGill University, ⁷Department of Medicine, Universite de Montreal - University of Montreal

This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.

Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

Estelle Rousselet¹, Jasna Kriz², Nabil G. Seidah¹

¹Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, ²CHUQ Research Centre, Laval University

The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers.

Medicine

In utero Measurement of Heart Rate in Mouse by Noninvasive M-mode Echocardiography

WooJin Kim¹, Nabil G. Seidah¹, Annik Prat¹

¹Laboratory of Biochemical Neuroendocrinology, Institut de Recherches Cliniques de Montréal

Ultra-high frequency ultrasound is a powerful live imaging tool to examine cardiac abnormalities in small animals. Its noninvasiveness allows maintaining the physiologic state of embryos. Herein, we show the use of M-mode ultrasound to measure the heart rates of embryos at E18.5 in utero.