로그인

Institut de recherches cliniques de Montréal

3 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
Sébastien D. Langlois *1,2, Steves Morin *1, Patricia T. Yam 1,3,4, Frédéric Charron 1,2,5,6,7
1Molecular Biology of Neural Development, Institut de Recherches Cliniques de Montréal, 2Division of Experimental Medicine and Program in Neuroengineering, McGill University, 3Program in Neuroengineering, McGill University, 4Montreal Neurological Institute, 5Department of Anatomy and Cell Biology, McGill University, 6Department of Biology, McGill University, 7Department of Medicine, Universite de Montreal - University of Montreal

This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.

image

Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining
Estelle Rousselet 1, Jasna Kriz 2, Nabil G. Seidah 1
1Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 2CHUQ Research Centre, Laval University

The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers.

image

Medicine

In utero Measurement of Heart Rate in Mouse by Noninvasive M-mode Echocardiography
WooJin Kim 1, Nabil G. Seidah 1, Annik Prat 1
1Laboratory of Biochemical Neuroendocrinology, Institut de Recherches Cliniques de Montréal

Ultra-high frequency ultrasound is a powerful live imaging tool to examine cardiac abnormalities in small animals. Its noninvasiveness allows maintaining the physiologic state of embryos. Herein, we show the use of M-mode ultrasound to measure the heart rates of embryos at E18.5 in utero.

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2024 MyJoVE Corporation. 판권 소유