Acute Kidney Injury Model Induced by Cisplatin in Adult ZebrafishCamila Morales Fénero *1, Barbara Nunes Padovani *1, Mariana Abrantes do Amaral *1,2, Guilherme José Bottura de Barros 1, Izabella Karina Xavier de Oliveira 1, Meire Ioshie Hiyane 1, Niels Olsen Saraiva Camâra 1,2
1Department of Immunology, University of São Paulo, 2Department of Medicine, Nephrology Division, Federal University of São Paulo
This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.
