Federal University of São Paulo

2 ARTICLES PUBLISHED IN JoVE

Medicine

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Camila Morales Fénero^*1, Barbara Nunes Padovani^*1, Mariana Abrantes do Amaral^*1,2, Guilherme José Bottura de Barros¹, Izabella Karina Xavier de Oliveira¹, Meire Ioshie Hiyane¹, Niels Olsen Saraiva Camâra^1,2

¹Department of Immunology, University of São Paulo, ²Department of Medicine, Nephrology Division, Federal University of São Paulo

This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.

Biology

A Swimming-Induced Zebrafish Exercise Apparatus for Versatile Training Approaches

Mauro Eugênio Medina Nunes¹, Lucas Herinque Silva do Carmo², Alexandre Budu^1,2, Ronaldo Carvalho Araujo^1,2

¹Exercise Genetics and Metabolism Laboratory, Molecular Biology Program, Department of Biophysics, Federal University of São Paulo, ²Exercise Genetics and Metabolism Laboratory, Nephrology Program, Department of Biophysics, University Federal of Sao Paulo

The exercise apparatus designed for less fortunate fish facilitates the implementation of diverse exercise protocols with varying intensities by manipulating water flow speed, achievable through rheotaxis.