École Normale Supérieure de Lyon

2 ARTICLES PUBLISHED IN JoVE

Genetics

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Diedre Reitz¹, Jérôme Savocco², Aurèle Piazza², Wolf-Dietrich Heyer^1,3

¹Department of Microbiology & Molecular Genetics, University of California, Davis, ²Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, ³Department of Molecular & Cellular Biology, University of California, Davis

The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.

Developmental Biology

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

Carla Guzman-Moreno^*1, Peizhao Zhang^*2, Olivia R. Phillips^*1, Mathias Block^*3, Benjamin J. Glennon^*1, Meghan Holbrook², Lauren Weigand², Cecilia W. Lo¹, Jiuann-Huey I. Lin^1,4

¹Department of Developmental Biology, University of Pittsburgh, ²Department of Biological Sciences, University of Pittsburgh, ³Département de Biologie, École Normale Supérieure de Lyon, ⁴Department of Critical Care Medicine, University of Pittsburgh

This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.