École Normale Supérieure de Lyon
2 ARTICLES PUBLISHED IN JoVE
Genetics
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Diedre Reitz 1, Jérôme Savocco 2, Aurèle Piazza 2, Wolf-Dietrich Heyer 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis
The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.
Developmental Biology
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
Carla Guzman-Moreno *1, Peizhao Zhang *2, Olivia R. Phillips *1, Mathias Block *3, Benjamin J. Glennon *1, Meghan Holbrook 2, Lauren Weigand 2, Cecilia W. Lo 1, Jiuann-Huey I. Lin 1,4
1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Département de Biologie, École Normale Supérieure de Lyon, 4Department of Critical Care Medicine, University of Pittsburgh
This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.
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