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University of Arizona College of Medicine

11 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice
Janet Alder 1, Wendy Fujioka 1, Jonathan Lifshitz 2,3, David P. Crockett 1, Smita Thakker-Varia 1
1Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 2Spinal Cord and Brain Injury Research Center, 3Department of Anatomy and Neurobiology, Department of Physical Medicine and Rehabilitation, University of Kentucky Chandler Medical Center

Lateral fluid percussion (LFP), an established model of traumatic brain injury in mice, is demonstrated. LFP fulfills three major criteria for animal models: validity, reliability and clinical relevance. The procedure, consisting of surgical craniotomy, fixation of hub followed by induction of injury, resulting in focal and diffuse injuries, is described.

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Medicine

The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis
Rishu Gupta 1,2, Maya Debbaneh 2,3, Daniel Butler 2,4, Monica Huynh 2,5, Ethan Levin 2, Argentina Leon 2, John Koo 2, Wilson Liao 2
1Keck School of Medicine, University of Southern California, 2Psoriasis and Skin Treatment Center Dermatology, University of California, San Francisco , 3University of California Irvine School of Medicine, 4University of Arizona College of Medicine, 5Chicago College of Osteopathic Medicine

Psoriasis is a chronic, immune-mediated inflammatory skin disease. The Goeckerman regimen, formulated for the treatment of psoriasis, consists of exposure to ultraviolet B (UVB) light and application of crude coal tar (CCT). The following protocol is for the administration of Goeckerman therapy for the treatment of moderate-to-severe psoriasis.

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Medicine

A Method to Study the Impact of Chemically-induced Ovarian Failure on Exercise Capacity and Cardiac Adaptation in Mice
Hao Chen 1, Jessica N. Perez 1, Eleni Constantopoulos 1, Laurel McKee 1, Jessica Regan 1, Patricia B. Hoyer 1, Heddwen L. Brooks 1, John Konhilas 1
1Department of Physiology, University of Arizona

Two exercise paradigms were tested on a newly developed chemically induced menopausal mouse model to examine the impact of menopause on exercise capacity and cardiac adaption to exercise.

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Neuroscience

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
Megan N. Evilsizor 1,2, Helen F. Ray-Jones 1,3, Jonathan Lifshitz 1,2,4,5, Jenna Ziebell 1,2
1Department of Child Health, University of Arizona College of Medicine - Phoenix, 2BARROW Neurological Institute, Phoenix Children's Hospital, 3Department of Biology and Biochemistry, University of Bath, 4Neuroscience Program, Arizona State University, 5Phoenix VA Healthcare System

This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brains, using markers for microglia and neuronal elements as an example.

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Biology

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
Pasano Bojang 1, Kenneth S. Ramos 1,2
1Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Arizona College of Medicine, 2Center for Applied Genetics and Genomic Medicine, University of Arizona College of Medicine

Here we employ FISH methodology to track LINE-1 retrotransposition at the single nuclei level in chromosome spreads of HepG2 cell lines stably expressing synthetic LINE-1.

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Medicine

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection
Hao Chen 1, Robert Klein 1, Stacy Arnold 1, Yiying Wang 2, Setsuko Chambers 3,4, Wenxin Zheng 5,6
1Department of Pathology, University of Arizona College of Medicine, 2Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, 3Department of Obstetrics and Gynecology, University of Arizona College of Medicine, 4University of Arizona Cancer Center, 5Department of Pathology, University of Texas Southwestern Medical Center, 6Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center

We explored a tubal cytologic method by sampling the fallopian tube directly post-surgical excision as a tool of ovarian cancer early detection. Here, we present a protocol to collect fallopian tube cells from freshly received surgical specimens.

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Biology

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
Zhen Wang 1,4, Ramon Ocadiz-Ruiz 1, Sinju Sundaresan 1, Lin Ding 1, Michael Hayes 1, Nirakar Sahoo 3, Haoxing Xu 1,2, Juanita L. Merchant 1,2,5
1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine

Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis.

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Cancer Research

Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System
Hao Chen *1, Thomas M.B. Ware *1, Josephine Iaria 1, Hong-Jian Zhu 1
1Department of Surgery (RMH), University of Melbourne

Here, we present a protocol for live cell imaging of TGF-β/Smad3 signaling activity using an adenovirus reporter system. This system tracks transcriptional activity in real-time and can be applied to both single cells in vitro and in live animalmodels.

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Neuroscience

Simultaneous Cryosectioning of Multiple Rodent Brains
Tabitha R.F. Green 1,2,3, J. Bryce Ortiz 1, Jordan L. Harrison 5, Jonathan Lifshitz 1,3,4, Rachel K. Rowe 1,3,4
1Department of Child Health, University of Arizona College of Medicine, 2Department of Biological Sciences, University of Bath, 3BARROW Neurological Institute at Phoenix Children's Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences, University of Arizona College of Medicine

Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging.

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JoVE Core

Setup and Execution Of the Blindfolded Code Training Exercise
Patrick G. Hughes 1, Kate E. Hughes 2, Rami A. Ahmed 3
1Florida Atlantic University Schmidt College of Medicine, 2Department of Emergency Medicine, University of Arizona College of Medicine, 3Department of Emergency Medicine, Indiana University School of Medicine

The blindfolded code training exercise, which blindfolds the team leader in a code resuscitation simulation, is an advanced teaching technique to improve closed-loop communication, organizational skills, and critical thinking. 

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JoVE Core

Setup and Execution of the Rapid Cycle Deliberate Practice Death Notification Curriculum
Patrick G. Hughes 1, Kate E. Hughes 2, Mary J. Hughes 3,4, Lindsay Weaver 4, Lauren E. Falvo 4, Anna M. Bona 4, Dylan Cooper 4, Cherri Hobgood 4, Rami A. Ahmed 4
1Florida Atlantic University Schmidt College of Medicine, 2Department of Emergency Medicine, University of Arizona College of Medicine, 3Department of Osteopathic Medical Specialties, College of Osteopathic Medicine, Michigan State University, 4Indiana University School of Medicine

The goal is to demonstrate how to apply the rapid cycle deliberate practice debriefing technique to the GRIEV_ING death notification curriculum.

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