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Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

DOI :

10.3791/53753-v

April 23rd, 2016

April 23rd, 2016

8,197 Views

1Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, University of Arizona College of Medicine, 2Center for Applied Genetics and Genomic Medicine, University of Arizona College of Medicine

Here we employ FISH methodology to track LINE-1 retrotransposition at the single nuclei level in chromosome spreads of HepG2 cell lines stably expressing synthetic LINE-1.

Tags

LINE 1 Retrotransposition

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