Tecnológico Nacional de México/Instituto Tecnológico de Morelia

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Bergmeyer Glucose Quantification for Microbiological Samples

Itan Homero Ruiz-Hernandez¹, Luis Alberto Madrigal-Perez², Christian Alexander Aburto-Tellez¹, Juan Carlos González-Hernández¹

¹Tecnológico Nacional de México/Instituto Tecnológico de Morelia, ²Tecnológico Nacional de México/Instituto Tecnológico Superior de Ciudad Hidalgo

Bergmeyer glucose quantification is a spectrophotometric enzymatic technique mainly used for clinical tests that accurately and sensitively measure the amount of glucose. Herein, we present a protocol for using this method for microbiological samples.

Biochemistry

Mitochondrial Respiration Quantification in Yeast Whole Cells

Gerardo M. Nava¹, Andres Carrillo-Garmendia¹, Juan Carlos González-Hernández², Luis Alberto Madrigal-Perez³

¹School of Chemistry, Universidad Autónoma de Querétaro, ²Department of Biochemical Engineering, Tecnológico Nacional de México/Instituto Tecnológico de Morelia, ³Department of Biochemical Engineering, Tecnológico Nacional de México/Instituto Tecnológico Superior de Ciudad Hidalgo

Mitochondrial respiration in yeast whole cells is a valuable indicator of cell bioenergetics. Here, we present a protocol to quantify this phenotype applicable to different yeast species.